猫丝孢菌对绝经后骨质疏松症的治疗作用：对肠道微生物群、代谢产物和免疫反应的调节

Therapeutic effects of Isaria felina on postmenopausal osteoporosis: modulation of gut microbiota, metabolites, and immune responses.

作者信息

Li Xiaoyan, Xue Chenhui, Yang Yongming, Zhao Lili, Chen Lixia, Wang Jing, Yan Lei, Meng Zan, Qiao Xiaochen, Liang Sujiao, Yang Xihua

机构信息

Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, China.

Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, Shanxi, China.

出版信息

Front Immunol. 2025 Apr 9;16:1508634. doi: 10.3389/fimmu.2025.1508634. eCollection 2025.

DOI:10.3389/fimmu.2025.1508634

PMID:40270955

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12015163/

Abstract

BACKGROUND

The intricate relationship between human health and gut microecology has emerged as a central theme in contemporary medical research. Postmenopausal osteoporosis, primarily driven by estrogen deficiency, remains a major health concern. Traditional Chinese herbal medicines have attracted significant interest for their promising role in osteoporosis treatment.

METHODS

The effects of , derived from , on postmenopausal osteoporosis in rats are the focus of this study. Adult female Sprague-Dawley rats were categorized into control, postmenopausal osteoporosis (OVX), and -treated (IF+OVX) groups. Following a 12-week treatment period, various analyses, including micro-CT, histological assessments, 16S rDNA sequencing, untargeted metabolomics, flow cytometry, and ELISA, were performed.

RESULTS

Micro-CT and histological assessments indicated significant improvements in bone loss and obesity control in OVX rats treated with . 16S rDNA sequencing revealed that corrected gut microbiota dysbiosis, particularly in the and . Untargeted metabolomics highlighted alterations in nucleotide and lipid metabolism. Flow cytometry and ELISA analyses demonstrated that modulated the Th17/Treg immune balance, resulting in reduced levels of inflammatory cytokines IL-17 and TNF-α.

CONCLUSIONS

These findings indicate that mitigates bone loss in postmenopausal osteoporosis through modulation of gut microbiota and immune responses, underscoring its potential as a therapeutic agent for osteoporosis treatment.

摘要

背景

人类健康与肠道微生态之间的复杂关系已成为当代医学研究的核心主题。绝经后骨质疏松症主要由雌激素缺乏引起，仍然是一个主要的健康问题。传统中草药因其在骨质疏松症治疗中的潜在作用而引起了广泛关注。

方法

本研究重点关注源自[具体来源]的[具体物质]对大鼠绝经后骨质疏松症的影响。成年雌性Sprague-Dawley大鼠被分为对照组、绝经后骨质疏松症（去势，OVX）组和[具体物质]治疗组（IF+OVX）。经过12周的治疗期后，进行了各种分析，包括显微CT、组织学评估、16S rDNA测序、非靶向代谢组学、流式细胞术和酶联免疫吸附测定（ELISA）。

结果

显微CT和组织学评估表明，用[具体物质]治疗的OVX大鼠在骨质流失和肥胖控制方面有显著改善。16S rDNA测序显示，[具体物质]纠正了肠道微生物群失调，特别是在[具体菌属1]和[具体菌属2]中。非靶向代谢组学突出了核苷酸和脂质代谢的改变。流式细胞术和ELISA分析表明，[具体物质]调节了Th17/Treg免疫平衡，导致炎症细胞因子IL-17和TNF-α水平降低。