Boot Kees J M, Hille Sander C, Korthout Henrie A A J, Libbenga Kees R, van Duijn Bert
Plant Biodynamics Laboratory, Institute of Biology, Leiden University, 2333 BE, Leiden, the Netherlands; Fytagoras, 2333 BE, Leiden, the Netherlands.
Plant Biodynamics Laboratory, Institute of Biology, Leiden University, 2333 BE, Leiden, the Netherlands; Mathematical Institute, Leiden University, 2333 CA, Leiden, the Netherlands.
J Plant Physiol. 2021 Jul;262:153436. doi: 10.1016/j.jplph.2021.153436. Epub 2021 May 15.
Measuring polar auxin transport (PAT) in plants and drawing conclusions from the observed transport data is only meaningful if these data are being analysed with a mathematical model which describes PAT. In this report we studied the polar auxin transport in Panax ginseng stems of different age and grown on different substrates.
We measured polar IAA transport in stems using a radio labelled IAA and analysed the transport data with a mathematical model we developed for Arabidopsis.
We found that PAT in ginseng stems, as compared to Arabidopsis inflorescence stems, has a 2-fold lower transport velocity and a 3-fold lower steady state auxin flux.
We were able to pinpoint two physiological parameters that influenced the observed transport characteristics in ginseng which differ from Arabidopsis, namely an increase in immobilization together with a reduced reflux of IAA from the surrounding tissue back to the transporting cells.
只有当植物中的极性生长素运输（PAT）数据通过描述PAT的数学模型进行分析时，测量PAT并从观察到的运输数据得出结论才有意义。在本报告中，我们研究了不同年龄且生长在不同基质上的人参茎中的极性生长素运输。
我们使用放射性标记的吲哚乙酸（IAA）测量茎中的极性IAA运输，并使用我们为拟南芥开发的数学模型分析运输数据。
我们发现，与人参花序茎相比，人参茎中的PAT运输速度低2倍，稳态生长素通量低3倍。
我们能够确定两个人参中影响观察到的运输特性的生理参数，这些参数与拟南芥不同，即固定化增加以及IAA从周围组织回流到运输细胞的减少。
