通过用干扰素预处理来阻断小鼠巨噬细胞中干扰素的合成。

Blocking of interferon synthesis in murine macrophages by pretreatment with interferon.

作者信息

Giese M, Kirchner H

机构信息

Institute of Virus Research, German Cancer Research Center, Heidelberg.

出版信息

Immunol Lett. 1988 Jun;18(2):109-13. doi: 10.1016/0165-2478(88)90049-1.

DOI:10.1016/0165-2478(88)90049-1

PMID:3403000

Abstract

The influence of pretreatment with interferon (IFN) on subsequent IFN synthesis was investigated in macrophage cultures of DBA/2 and C57BL/6 mice. The doses of IFN alpha/beta for pretreatment ranged from 10,000 U/ml to 100 U/ml and the incubation time was between 18 and 2 h. No blocking effect was observed for chemical induction with poly I:poly C or CMA. However, for viral infection with NDV, blocking was observed. This inhibition of IFN synthesis was dependent on the dose and time of IFN pretreatment and of the titer of the inducing virus. Similarly in mouse fibroblast cultures no blocking activity was observed for induction with poly I:poly C/DEAE-dextran. Again, with NDV as inducer, pretreatment with IFN resulted in inhibition of interferon synthesis. Thus, our data show that blocking occurs only with a viral inducer and suggest that it is caused by an antiviral effect.

摘要

在DBA/2和C57BL/6小鼠的巨噬细胞培养物中，研究了用干扰素（IFN）预处理对随后IFN合成的影响。用于预处理的IFNα/β剂量范围为10,000 U/ml至100 U/ml，孵育时间为18至2小时。对于用聚肌苷酸：聚胞苷酸（poly I:poly C）或化学激活剂（CMA）进行的化学诱导，未观察到阻断作用。然而，对于新城疫病毒（NDV）感染，观察到了阻断现象。这种对IFN合成的抑制取决于IFN预处理的剂量和时间以及诱导病毒的滴度。同样，在小鼠成纤维细胞培养物中，用聚肌苷酸：聚胞苷酸/二乙氨基乙基葡聚糖（poly I:poly C/DEAE-dextran）诱导时未观察到阻断活性。再次，以NDV作为诱导剂，用IFN预处理导致干扰素合成受到抑制。因此，我们的数据表明，仅在病毒诱导剂作用下才会发生阻断，并提示这是由抗病毒效应引起的。