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齿双歧杆菌的代谢特征反映了其作为人类肠道共生菌的状态。

The metabolic profile of Bifidobacterium dentium reflects its status as a human gut commensal.

机构信息

Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, USA.

Department of Pathology, Texas Children's Hospital, Houston, TX, USA.

出版信息

BMC Microbiol. 2021 May 24;21(1):154. doi: 10.1186/s12866-021-02166-6.

DOI:10.1186/s12866-021-02166-6
PMID:34030655
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8145834/
Abstract

BACKGROUND

Bifidobacteria are commensal microbes of the mammalian gastrointestinal tract. In this study, we aimed to identify the intestinal colonization mechanisms and key metabolic pathways implemented by Bifidobacterium dentium.

RESULTS

B. dentium displayed acid resistance, with high viability over a pH range from 4 to 7; findings that correlated to the expression of Na+/H+ antiporters within the B. dentium genome. B. dentium was found to adhere to human MUC2+ mucus and harbor mucin-binding proteins. Using microbial phenotyping microarrays and fully-defined media, we demonstrated that in the absence of glucose, B. dentium could metabolize a variety of nutrient sources. Many of these nutrient sources were plant-based, suggesting that B. dentium can consume dietary substances. In contrast to other bifidobacteria, B. dentium was largely unable to grow on compounds found in human mucus; a finding that was supported by its glycosyl hydrolase (GH) profile. Of the proteins identified in B. dentium by proteomic analysis, a large cohort of proteins were associated with diverse metabolic pathways, indicating metabolic plasticity which supports colonization of the dynamic gastrointestinal environment.

CONCLUSIONS

Taken together, we conclude that B. dentium is well adapted for commensalism in the gastrointestinal tract.

摘要

背景

双歧杆菌是哺乳动物胃肠道的共生微生物。在这项研究中，我们旨在确定双歧杆菌定植肠道的机制和关键代谢途径。

结果

双歧杆菌具有耐酸性，在 pH 值为 4 到 7 的范围内具有高存活率；这一发现与双歧杆菌基因组中存在的 Na+/H+反向转运蛋白的表达相关。双歧杆菌被发现可以黏附在人 MUC2+黏液上，并携带黏蛋白结合蛋白。通过使用微生物表型微阵列和完全定义的培养基，我们证明在没有葡萄糖的情况下，双歧杆菌可以代谢各种营养源。其中许多营养源是植物来源的，这表明双歧杆菌可以消耗膳食物质。与其他双歧杆菌不同，双歧杆菌在很大程度上无法在人黏液中发现的化合物上生长；这一发现得到了其糖苷水解酶 (GH) 图谱的支持。通过蛋白质组学分析鉴定出的双歧杆菌中的蛋白质，其中很大一部分与多种代谢途径相关，表明其具有支持定植在动态胃肠道环境中的代谢可塑性。

结论

综上所述，我们得出结论，双歧杆菌非常适合在胃肠道中作为共生菌存在。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/522c49a9ecb8/12866_2021_2166_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/d4a3cfb6707c/12866_2021_2166_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/27e40d3d81fa/12866_2021_2166_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/ff998d6fcac0/12866_2021_2166_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/f3602172e76c/12866_2021_2166_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/e2d9ba8f5676/12866_2021_2166_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/5a042554c9e3/12866_2021_2166_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/7ae3484611dd/12866_2021_2166_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/9d35c137abce/12866_2021_2166_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/522c49a9ecb8/12866_2021_2166_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/d4a3cfb6707c/12866_2021_2166_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/27e40d3d81fa/12866_2021_2166_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/ff998d6fcac0/12866_2021_2166_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/f3602172e76c/12866_2021_2166_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/e2d9ba8f5676/12866_2021_2166_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/5a042554c9e3/12866_2021_2166_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/7ae3484611dd/12866_2021_2166_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/9d35c137abce/12866_2021_2166_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9710/8145834/522c49a9ecb8/12866_2021_2166_Fig9_HTML.jpg

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本文引用的文献

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Cell Mol Gastroenterol Hepatol. 2021;11(1):221-248. doi: 10.1016/j.jcmgh.2020.08.002. Epub 2020 Aug 12.
3
Bifidobacteria shape host neural circuits during postnatal development by promoting synapse formation and microglial function.
基于培养的炎症性肠病肠道微生物群特征分析
Front Microbiol. 2025 Feb 20;16:1538620. doi: 10.3389/fmicb.2025.1538620. eCollection 2025.
4
Synergistic Effects of Probiotics and Lifestyle Interventions on Intestinal Microbiota Composition and Clinical Outcomes in Obese Adults.益生菌与生活方式干预对肥胖成年人肠道微生物群组成及临床结局的协同作用
Metabolites. 2025 Jan 23;15(2):70. doi: 10.3390/metabo15020070.
5
Investigation of the nutritional and functional roles of a microencapsulated blend of botanicals on intestinal health and growth of nursery pigs challenged with F18+Escherichia coli.研究微囊化植物提取物混合物对受F18 + 大肠杆菌攻击的保育猪肠道健康和生长的营养及功能作用。
J Anim Sci. 2025 Jan 4;103. doi: 10.1093/jas/skaf047.
6
Taxonomic and phenotypic analysis of bifidobacteria isolated from IBD patients as potential probiotic strains.从 IBD 患者中分离的双歧杆菌的分类和表型分析作为潜在的益生菌菌株。
BMC Microbiol. 2024 Jun 29;24(1):233. doi: 10.1186/s12866-024-03368-4.
7
The role of genus in modulating the neonate microbiota: implications for antibiotic resistance acquisition in early life.属在调节新生儿微生物群中的作用：对生命早期抗生素耐药性获得的影响。
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8
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9
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4
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5
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6
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