Am J Vet Res. 2021 Jun;82(6):478-486. doi: 10.2460/ajvr.82.6.478.
To investigate the in vitro effects of clinically relevant concentrations of the local anesthetics (LAs) bupivacaine, lidocaine, lidocaine with preservative (LP), mepivacaine, and ropivacaine on equine chondrocyte and fibroblast-like synoviocyte (FLS) viability.
Chondrocytes and FLSs of the metacarpophalangeal joints of 4 healthy adult horses.
Viability of chondrocytes and FLSs was determined with 3 assays: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH), and trypan blue (TB) exclusion (only FLS). Viability was assessed after 30- and 60-minute exposures to 0.0625%, 0.125%, and 0.25% bupivacaine; 0.25%, 0.5%, and 1% lidocaine; 0.25%, 0.5%, and 1% LP; 0.25%, 0.5%, and 1% mepivacaine; and 0.125%, 0.25%, and 0.5% ropivacaine.
Viability of chondrocytes was significantly decreased with exposure to 0.25% bupivacaine, 1% lidocaine, 1% LP, 1% mepivacaine, and 0.25% ropivacaine. Viability of FLSs was significantly decreased with exposure to 0.25% bupivacaine, 1% mepivacaine, 1% LP, and 0.5% ropivacaine.
Clinically relevant concentrations of LAs had in vitro time- and concentration-dependent cytotoxicity for chondrocytes and FLSs isolated from the metacarpophalangeal joints of healthy horses. Bupivacaine was more toxic to chondrocytes than lidocaine, mepivacaine, and ropivacaine, whereas bupivacaine, LP, mepivacaine, and ropivacaine were more toxic to FLSs than preservative-free lidocaine. Several LAs may negatively affect chondrocyte and FLS viability.
研究临床相关浓度的局部麻醉剂（LA）布比卡因、利多卡因、含防腐剂的利多卡因（LP）、甲哌卡因和罗哌卡因对马关节软骨细胞和成纤维样滑膜细胞（FLS）活力的体外影响。
来自 4 匹健康成年马掌指关节的软骨细胞和 FLS。
通过 3 种方法测定软骨细胞和 FLS 的活力：3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）、乳酸脱氢酶（LDH）和台盼蓝（TB）排除（仅 FLS）。在 30 至 60 分钟内暴露于 0.0625%、0.125%和 0.25%布比卡因、0.25%、0.5%和 1%利多卡因、0.25%、0.5%和 1%LP、0.25%、0.5%和 1%甲哌卡因和 0.125%、0.25%和 0.5%罗哌卡因后，评估活力。
暴露于 0.25%布比卡因、1%利多卡因、1%LP、1%甲哌卡因和 0.25%罗哌卡因可显著降低软骨细胞活力。暴露于 0.25%布比卡因、1%甲哌卡因、1%LP 和 0.5%罗哌卡因可显著降低 FLS 活力。
临床相关浓度的 LA 对来自健康马掌指关节的软骨细胞和成纤维样滑膜细胞具有体外时间和浓度依赖性细胞毒性。与利多卡因、甲哌卡因和罗哌卡因相比，布比卡因对软骨细胞的毒性更大，而布比卡因、LP、甲哌卡因和罗哌卡因对 FLS 的毒性大于不含防腐剂的利多卡因。几种 LA 可能会对软骨细胞和成纤维样滑膜细胞的活力产生负面影响。
