Kuhn A, Mahrle G, Steigleder G K
Universitäts-Hautklinik Köln.
Hautarzt. 1988 Jun;39(6):351-4.
In a study on 26 patients with autoimmune diseases the immunostaining for immunoglobulins and complement in frozen sections was compared with that in formalin-fixed and paraffin-embedded sections. The formalin-fixed material of pemphigus vulgaris, lupus erythematosus, bullous pemphigoid, and dermatitis herpetiformis (Duhring's disease) was reconstituted and stained by means of the standard two-step technique (TST), the peroxidase-antiperoxidase technique (PAP), and the streptavidin-biotin method (SAB). In comparison with frozen sections, immunoglobulins and complement could also be demonstrated in formalin-fixed sections in all cases of pemphigus vulgaris and in 85% of cases of discoid lupus erythematosus, but in only 60% of cases of bullous pemphigoid or Duhring's disease. PAP and SAB proved to be about equally reliable, but TST was significantly less dependable.
在一项针对26例自身免疫性疾病患者的研究中，对冰冻切片中免疫球蛋白和补体的免疫染色与福尔马林固定石蜡包埋切片中的免疫染色进行了比较。对寻常型天疱疮、红斑狼疮、大疱性类天疱疮和疱疹样皮炎（杜林病）的福尔马林固定材料进行了重构，并采用标准两步法（TST）、过氧化物酶抗过氧化物酶技术（PAP）和链霉亲和素-生物素法（SAB）进行染色。与冰冻切片相比，在所有寻常型天疱疮病例以及85%的盘状红斑狼疮病例的福尔马林固定切片中也能检测到免疫球蛋白和补体，但在大疱性类天疱疮或杜林病病例中只有60%能检测到。结果证明PAP和SAB的可靠性大致相当，但TST的可靠性明显较低。
