Nisengard R J, Blaszczyk M, Chorzelski T, Beutner E
Arch Dermatol. 1978 Sep;114(9):1329-32.
Immunofluorescence findings for IgG, IgA, IgM, and C4 on biopsy specimens quick frozen and transported frozen were compared with findings on portions of the same biopsy specimens placed in holding solution and transported at ambient temperatures. A total of 52 biopsy specimens were examined from normal individuals and patients with systemic lupus erythematosus (SLE), discoid lupus erythematosus (DLE), pemphigus, pemphigoid, and dermatitis herpetiformis. Overall agreement of results was 90%: 88% in SLE; 50% to 66% in DLE, 96% in pemphigus; 92% in bullous pemphigoid; and 87% in dermatitis herpetiformis. Except for two of the 42 biopsy specimens, the combined IgG, IgA, IgM, and C4 findings were the same. In one DLE case, only the frozen biopsy specimen was positive. In one case of dermatitis herpetiformis, only the ambient temperature biopsy specimen was positive. Results indicate the holding solution at ambient temperature can be used in place of the frozen method.
将快速冷冻并在冷冻状态下运送的活检标本上IgG、IgA、IgM和C4的免疫荧光结果,与置于保存液中并在室温下运送的同一活检标本部分的结果进行比较。共检查了52份来自正常个体以及患有系统性红斑狼疮(SLE)、盘状红斑狼疮(DLE)、天疱疮、类天疱疮和疱疹样皮炎患者的活检标本。结果的总体一致性为90%:SLE中为88%;DLE中为50%至66%;天疱疮中为96%;大疱性类天疱疮中为92%;疱疹样皮炎中为87%。除42份活检标本中的两份外,IgG、IgA、IgM和C4的综合结果相同。在1例DLE病例中,仅冷冻活检标本呈阳性。在1例疱疹样皮炎病例中,仅室温活检标本呈阳性。结果表明,室温下的保存液可替代冷冻方法。