Bartels H, Vogt B, Jungermann K
Institut für Biochemie, Georg-August-Universität, Göttingen, Federal Republic of Germany.
Histochemistry. 1988;89(3):253-60. doi: 10.1007/BF00493149.
The isolated liver from 24 h fasted rats was perfused in a non-recirculating manner in the ortho- and retrograde direction with erythrocyte-containing (20% v/v) media to provide adequate oxygenation of the liver. Glucose and/or gluconeogenic precursors were added as substrates. Glycogen formation was determined biochemically and demonstrated histochemically. With glucose as the sole exogenous substrate glycogen was deposited in the perivenous area, with gluconeogenic precursors it was formed in the periportal zone during ortho- and retrograde flow. When glucose and gluconeogenic compounds were offered together, glycogen was deposited in both zones. The results corroborate the model of metabolic zonation predicting that periportal glycogen is synthesized indirectly from gluconeogenic precursors while perivenous glycogen is formed directly from glucose.
将禁食24小时大鼠的离体肝脏，用含红细胞（20% v/v）的培养基以非循环方式进行顺行和逆行灌注，以确保肝脏有充足的氧合。添加葡萄糖和/或糖异生前体作为底物。通过生化方法测定糖原生成，并进行组织化学证明。以葡萄糖作为唯一外源底物时，糖原沉积在肝静脉周围区域；以糖异生前体作为底物时，在顺行和逆行灌注过程中，糖原在门静脉周围区域形成。当同时提供葡萄糖和糖异生化合物时，糖原在两个区域均有沉积。这些结果证实了代谢分区模型，该模型预测门静脉周围的糖原是由糖异生前体间接合成的，而肝静脉周围的糖原是由葡萄糖直接形成的。
