Tal M, Schneider D L, Thorens B, Lodish H F
Whitehead Institute For Biomedical Research, Cambridge, Massachusetts 02142.
J Clin Invest. 1990 Sep;86(3):986-92. doi: 10.1172/JCI114801.
The "erythroid/brain" glucose transporter (GT) isoform is expressed only in a subset of hepatocytes, those forming the first row around the terminal hepatic venules, while the "liver" GT is expressed in all hepatocytes. After 3 d of starvation, a three- to fourfold elevation of expression of the erythroid/brain GT mRNA and protein is detected in the liver as a whole; this correlates with the expression of this GT in more hepatocytes, those forming the first three to four rows around the hepatic venules. Starvation-dependent expression of the erythroid/brain GT on the plasma membrane of these additional hepatocytes is lost within 3 h of glucose refeeding; however, by immunoblotting we show that the protein is still present. Its loss from the surface is possibly explained by internalization.
“红细胞/脑”葡萄糖转运体（GT）同工型仅在肝细胞的一个亚群中表达，即那些围绕终末肝静脉形成第一排的肝细胞，而“肝脏”GT在所有肝细胞中均有表达。饥饿3天后，在整个肝脏中检测到红细胞/脑GT mRNA和蛋白的表达升高了三到四倍；这与该GT在更多肝细胞中的表达相关，即那些围绕肝静脉形成前三到四排的肝细胞。在重新喂食葡萄糖3小时内，这些额外肝细胞质膜上依赖饥饿的红细胞/脑GT表达丧失；然而，通过免疫印迹我们表明该蛋白仍然存在。其从表面的丧失可能是由于内化作用。
