原代人和啮齿动物脑微血管内皮细胞的分离：培养、鉴定和高效转染。
Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection.
机构信息
Department of Neurology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
Shriners Hospital Pediatric Research Center, Temple University School of Medicine, Philadelphia, PA, USA.
出版信息
Methods Mol Biol. 2021;2311:185-193. doi: 10.1007/978-1-0716-1437-2_15.
Studies of blood-brain barrier (BBB) require developing of a novel and convenient in vitro endothelial cell model. We isolated primary human and rodent brain microvascular endothelial cells and developed methods for culturing, characterization, and high-efficiency transfection of endothelial cells. Here, we describe the improved methods to obtain in vitro human and rodent BBB models to study expression of endogenous and exogenous genes of interest.
研究血脑屏障（BBB）需要开发一种新颖、方便的体外内皮细胞模型。我们分离了原代人源和啮齿动物脑微血管内皮细胞，并开发了培养、鉴定和高效转染内皮细胞的方法。在这里，我们描述了改进的方法来获得体外人源和啮齿动物 BBB 模型，以研究内源性和外源性目的基因的表达。
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