用于体外血脑屏障研究的脑微血管内皮细胞的分离与培养。

Isolation and Culture of Brain Microvascular Endothelial Cells for In Vitro Blood-Brain Barrier Studies.

作者信息

Rosas-Hernandez Hector, Cuevas Elvis, Lantz Susan M, Paule Merle G, Ali Syed F

机构信息

Division of Neurotoxicology, HFT-132, National Center for Toxicological Research/USFDA, Jefferson, AR, USA.

出版信息

Methods Mol Biol. 2018;1727:315-331. doi: 10.1007/978-1-4939-7571-6_23.

DOI:10.1007/978-1-4939-7571-6_23

PMID:29222791

Abstract

The blood-brain barrier (BBB) is essential to maintain the proper microenvironment for brain function. Although formed by different cell types, the endothelial cells (ECs) of the brain microvessels provide the BBB with its selective permeability. To study the BBB in vitro, EC lines as well as primary isolated ECs have been used. In this chapter, we will provide a detailed protocol on how to isolate and culture primary brain microvascular endothelial cells from different species for use as in vitro models of the BBB. When performed properly, this protocol will allow one to obtain a pure culture of brain microvascular endothelial cells with which to analyze the effects of therapeutic and toxic agents on BBB functions.

摘要

血脑屏障（BBB）对于维持大脑功能的适当微环境至关重要。尽管血脑屏障由不同类型的细胞形成，但脑微血管的内皮细胞（ECs）赋予了血脑屏障选择性通透性。为了在体外研究血脑屏障，人们使用了内皮细胞系以及原代分离的内皮细胞。在本章中，我们将提供一份详细的方案，介绍如何从不同物种中分离和培养原代脑微血管内皮细胞，以用作血脑屏障的体外模型。如果操作得当，该方案将使人们能够获得脑微血管内皮细胞的纯培养物，用以分析治疗剂和毒性剂对血脑屏障功能的影响。