Department for Clinical Sciences B67, Faculty of Veterinary Medicine, Université de Liège, Quartier Vallée 2, B44, Boulevard de Colonster 3, Liège, 4000, Belgium.
Laboratory of Food Microbiology, Fundamental and applied Research for Animals and Health center (FARAH), Université de Liège, Quartier Vallée 2, B42, Avenue de Cureghem 180, Liège, 4000, Belgium.
Vet Dermatol. 2021 Aug;32(4):355-e98. doi: 10.1111/vde.12977. Epub 2021 May 25.
Otitis externa is associated with a lack of bacterial/fungal diversity in atopic dermatitis. Clinical experience has shown that use of topical corticosteroids in the ear canal (EC) can prevent otitis. No data are available on the impact of this treatment on the EC microbiota.
HYPOTHESIS/OBJECTIVES: To observe the bacterial/fungal diversity in the EC and the clinical effect of topical corticosteroids administered over a four week period in atopic dogs without active otitis.
Ten atopic dogs without active otitis.
Mometasone was applied in the right EC, while the left was used as control. A clinical and cytological evaluation of the EC was performed. Swabs of each EC were analysed using next-generation sequencing methods.
At the beginning of the trial, variations in microbiota and mycobiota were observed between dogs and also within individuals. Statistically, no significant difference was observed in alpha and beta diversity between the treated and the untreated group over time. Clinically, right and left EC diversities were no different at Day (D)28 (P = 0.28). A significant difference was noted between D0 and D28 for the treated ears (P = 0.012) and not for the untreated ears (P = 0.63). No cytological evidence of microbes was found for treated ECs at D28.
These data suggest that the use of topical corticosteroids as proactive treatment is unlikely to increase the risk of secondary microbial overgrowth. The positive clinical effect of this proactive treatment seems to be supported through cytological and otoscopic improvement.
在特应性皮炎中，外耳道与细菌/真菌多样性的缺乏有关。临床经验表明，在外耳道使用局部皮质类固醇可以预防中耳炎。目前尚无关于这种治疗方法对外耳道微生物群影响的数据。
假设/目的：观察无活动性中耳炎的特应性犬外耳道的细菌/真菌多样性，并评估在四周内局部使用皮质类固醇的临床效果。
10 只无活动性中耳炎的特应性犬。
右外耳道应用莫米松，而左侧作为对照。对外耳道进行临床和细胞学评估。对每个外耳道的拭子进行下一代测序分析。
在试验开始时，观察到犬之间以及个体内的微生物群和真菌群存在差异。从统计学上看，治疗组和未治疗组的 alpha 和 beta 多样性在整个时间内均无显著差异。临床方面，第 28 天右外耳道和左外耳道的多样性无差异（P=0.28）。治疗耳在第 0 天和第 28 天之间存在显著差异（P=0.012），而未治疗耳无差异（P=0.63）。第 28 天，治疗后的外耳道中未发现微生物的细胞学证据。
这些数据表明，局部皮质类固醇作为预防性治疗不太可能增加继发性微生物过度生长的风险。这种预防性治疗的积极临床效果似乎通过细胞学和耳镜改善得到支持。
