Leonard Caroline, Picavet Pierre P, Fontaine Jacques, Clercx Cécile, Taminiau Bernard, Daube Georges, Claeys Stéphanie
Department of Clinical Sciences, Faculty of Veterinary Medicine, B67 Sart Tilman, University of Liege, 4000 Liege, Belgium.
Fundamental and Applied Research for Animal & Health (FARAH), Food Science Department, Faculty of Veterinary Medicine, B43b Sart Tilman, University of Liege, 4000 Liege, Belgium.
Vet Sci. 2023 Mar 11;10(3):216. doi: 10.3390/vetsci10030216.
Otitis media can be a consequence of chronic otitis externa and could represent a perpetuating factor. While the microbiota of the EEC in healthy dogs and in the presence of otitis externa has been described, only sparse information is available concerning the normal microbiota of the middle ear. The objective was to compare the tympanic bulla (TB) with the external ear canal (EEC) microbiota in healthy dogs. Six healthy experimental Beagle dogs were selected based on the absence of otitis externa, negative cytology and bacterial culture from the TB. Samples from the EEC and TB were collected directly after death using a total ear canal ablation and lateral bulla osteotomy. The hypervariable segment V1-V3 of the 16S rDNA was amplified and sequenced with a MiSeq Illumina. The sequences were analyzed by the Mothur software using the SILVA database. No significant differences between the EEC and TB microbiota for the Chao1 richness index ( = 0.6544), the Simpson evenness index ( = 0.4328) and the reciprocal Simpson alpha diversity ( = 0.4313) were noted (Kruskal-Wallis test). A significant difference ( = 0.009) for the Chao1 richness index between the right and left EEC was observed. The microbiota profile was similar in the EEC and the TB of the Beagles.
中耳炎可能是慢性外耳道炎的结果，并且可能是一个持续存在的因素。虽然已经描述了健康犬以及患有外耳道炎时外耳道的微生物群，但关于中耳正常微生物群的信息却很少。目的是比较健康犬的鼓泡（TB）和外耳道（EEC）的微生物群。基于无外耳道炎、鼓泡细胞学检查阴性和细菌培养阴性，选择了6只健康的实验性比格犬。犬死亡后，通过全外耳道切除术和外侧鼓泡截骨术直接采集外耳道和鼓泡的样本。使用Illumina MiSeq对16S rDNA的高变区V1-V3进行扩增和测序。使用SILVA数据库通过Mothur软件对序列进行分析。对于Chao1丰富度指数（= 0.6544）、辛普森均匀度指数（= 0.4328）和倒数辛普森α多样性（= 0.4313），外耳道和鼓泡微生物群之间未发现显著差异（Kruskal-Wallis检验）。观察到左右外耳道的Chao1丰富度指数存在显著差异（= 0.009）。比格犬的外耳道和鼓泡中的微生物群谱相似。
