Walker D C, MacKenzie A L, Wiggs B R, Montaner J G, Hogg J C
Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, British Columbia, Canada.
J Appl Physiol (1985). 1988 Jun;64(6):2348-56. doi: 10.1152/jappl.1988.64.6.2348.
This study is intended to determine whether qualitative assessment of tight junction integrity from freeze-fracture data is reliable. We used lung parenchyma from a control mongrel dog's cardiac lung lobe, from a mongrel dog subjected to vascular high-pressure pulmonary edema (HPPE), and from a dog subjected to oleic acid-induced low-pressure pulmonary edema (LPPE) (6). Quantitative assessment was done on 115 freeze-fracture micrographs of epithelial tight junctions and on another 158 freeze-fracture micrographs of endothelial junctions from the 3 dogs. Quantitative assessment showed differences between the dogs in junction depth, fibril numbers, density, and complexity. for qualitative assessment, these same 273 micrographs were assessed in a single-blind fashion by having six investigators sort first the epithelial and then the endothelial junctions into normal or damaged categories. Qualitative assessment did not agree with quantitative data, suggesting that it is unreliable.
本研究旨在确定从冷冻断裂数据对紧密连接完整性进行定性评估是否可靠。我们使用了来自对照杂种犬心脏肺叶、患有血管性高压肺水肿（HPPE）的杂种犬以及患有油酸诱导的低压肺水肿（LPPE）的犬的肺实质（6）。对这3只犬的115张上皮紧密连接冷冻断裂显微照片和另外158张内皮连接冷冻断裂显微照片进行了定量评估。定量评估显示，这些犬在连接深度、纤维数量、密度和复杂性方面存在差异。对于定性评估，由6名研究人员以单盲方式对这273张显微照片进行评估，首先将上皮连接然后将内皮连接分为正常或受损类别。定性评估与定量数据不一致，表明其不可靠。
