Winn R, Nickelson S, Rice C L
Department of Physiology-Biophysics, University of Washington, Seattle 98195.
J Appl Physiol (1985). 1988 Jun;64(6):2463-7. doi: 10.1152/jappl.1988.64.6.2463.
The Starling fluid filtration coefficient (Kf) of blood-perfused excised goat lungs was examined before and after infusion of Escherichia coli endotoxin. Kf was calculated from rate of weight gain as described by Drake et al. [Am. J. Physiol. 234 (Heart Circ. Physiol. 3): H266-H274, 1978]. These calculations were made twice during base line and then at hourly intervals for 5 h after infusion of 5 mg (approximately 250 micrograms/kg) of E. coli endotoxin or after injection of oleic acid (47 microliter/kg). All lungs were perfused at constant arterial and venous pressure under zone 3 conditions. Base-line Kf averaged 27 +/- 10 and 20 +/- 4 (SD) microliter.min-1.cmH2O-1.g dry wt-1 for endotoxin and oleic acid groups, respectively. It was unchanged in the endotoxin group throughout the experiment but approximately doubled in the oleic acid lungs. Pulmonary arterial and venous pressures were not changed significantly during the course of these experiments in either group. Lung wet-to-dry weight ratios of these lungs were 5.6 +/- 0.6 and 6.1 +/- 0.5 ml/g for the endotoxin and oleic acid groups, respectively. This compares with 4.6 +/- 0.5 ml/g for normal, freshly excised but not perfused goat lungs. The small change in lung water and unchanged pulmonary pressures after both endotoxin and oleic acid suggest that lung injury was minimal. We conclude that 1) endotoxin does not cause a direct injury to the endothelium of isolated lungs during the first 5 h of perfusion, and 2) neutrophils are not sufficient to cause increased Kf after endotoxin infusion in this preparation.
在输注大肠杆菌内毒素前后，对血液灌注的离体山羊肺的斯塔林流体滤过系数（Kf）进行了检测。Kf是根据德雷克等人所述的体重增加率计算得出的[《美国生理学杂志》234卷（心脏循环生理学3）：H266 - H274，1978年]。在基线期进行了两次此类计算，然后在输注5毫克（约250微克/千克）大肠杆菌内毒素或注射油酸（47微升/千克）后的5小时内，每隔一小时进行一次计算。所有肺均在3区条件下，于恒定的动脉压和静脉压下进行灌注。内毒素组和油酸组的基线Kf分别平均为27±10和20±4（标准差）微升·分钟⁻¹·厘米水柱⁻¹·克干重⁻¹。在内毒素组中，整个实验过程中Kf未发生变化，但在油酸处理的肺中，Kf约增加了一倍。在这两组实验过程中，肺动脉压和静脉压均未发生显著变化。这些肺的肺湿重与干重之比，内毒素组和油酸组分别为5.6±0.6和6.1±0.5毫升/克。相比之下，正常的、刚切除但未灌注的山羊肺的该比值为4.6±0.5毫升/克。内毒素和油酸处理后肺水的微小变化以及肺压力未改变表明肺损伤最小。我们得出结论：1）在内毒素灌注的最初5小时内，内毒素不会对离体肺的内皮造成直接损伤；2）在该制剂中，中性粒细胞不足以导致内毒素输注后Kf升高。
