Department of Anesthesiology and Critical Care Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA.
Division of Nuclear Medicine and Molecular Imaging, The Russell H. Morgan Department of Radiology and Radiological Science, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA.
Sci Rep. 2021 May 25;11(1):10885. doi: 10.1038/s41598-021-90216-4.
The relationship between cerebral glucose metabolism and glucose transporter expression after intracerebral hemorrhage (ICH) is unclear. Few studies have used positron emission tomography (PET) to explore cerebral glucose metabolism after ICH in rodents. In this study, we produced ICH in mice with an intrastriatal injection of collagenase to investigate whether glucose metabolic changes in F-fluoro-2-deoxy-D-glucose (FDG)-PET images are associated with expression of glucose transporters (GLUTs) over time. On days 1 and 3 after ICH, the ipsilateral striatum exhibited significant hypometabolism. However, by days 7 and 14, glucose metabolism was significantly higher in the ipsilateral striatum than in the contralateral striatum. The contralateral hemisphere did not show hypermetabolism at any time after ICH. Qualitative immunofluorescence and Western blotting indicated that the expression of GLUT1 in ipsilateral striatum decreased on days 1 and 3 after ICH and gradually returned to baseline by day 21. The F-FDG uptake after ICH was associated with expression of GLUT1 but not GLUT3 or GLUT5. Our data suggest that ipsilateral cerebral glucose metabolism decreases in the early stage after ICH and increases progressively in the late stage. Changes in F-FDG uptake on PET imaging are associated with the expression of GLUT1 in the ipsilateral striatum.
脑出血 (ICH) 后大脑葡萄糖代谢与葡萄糖转运蛋白表达之间的关系尚不清楚。很少有研究使用正电子发射断层扫描 (PET) 来探索啮齿动物 ICH 后的大脑葡萄糖代谢。在这项研究中，我们通过纹状体内胶原酶注射在小鼠中产生 ICH，以研究 F-氟-2-脱氧-D-葡萄糖 (FDG)-PET 图像中的葡萄糖代谢变化是否与葡萄糖转运蛋白 (GLUTs) 的表达随时间而变化有关。在 ICH 后第 1 天和第 3 天，对侧纹状体表现出明显的低代谢。然而，在第 7 天和第 14 天，对侧纹状体的葡萄糖代谢明显高于对侧纹状体。在 ICH 后任何时间，对侧半球均未出现高代谢。定性免疫荧光和 Western blot 表明，ICH 后第 1 天和第 3 天对侧纹状体 GLUT1 的表达减少，并在第 21 天逐渐恢复到基线。ICH 后 F-FDG 的摄取与 GLUT1 的表达有关，但与 GLUT3 或 GLUT5 无关。我们的数据表明，ICH 后早期对侧大脑葡萄糖代谢减少，晚期逐渐增加。PET 成像上 F-FDG 摄取的变化与对侧纹状体 GLUT1 的表达有关。
