Maria Rahul, Dutta Smita D, Thete Sanjay G, AlAttas Mustafa Hussein
Department of Conservative Dentistry and Endodontics, Bhabha College of Dental Sciences, Bhopal, Madhya Pradesh, India.
Department of Conservative Dental Sciences and Endodontics, College of Dentistry, Qassim University, Buraydah, Saudi Arabia.
J Int Soc Prev Community Dent. 2021 Apr 15;11(2):179-183. doi: 10.4103/jispcd.JISPCD_422_20. eCollection 2021 Mar-Apr.
The use of solvents is necessary to remove remnants of filling materials within dentinal tubules to allow penetration of irrigating solution within the tubules.
We aimed at determining the antibacterial effect of various gutta-percha (GP) solvents against (E. faecalis).
An study was conducted by measuring the zone of inhibition using the disk diffusion method. The test organism used for the study was , and the solvents were divided into five groups: eucalyptus oil, chloroform, turpentine oil, xylene, and orange oil. About 500 μL of the suspension was spread over the agar plates, and the empty sterilized disks were impregnated with 10 μL of pure essential oils (EOs). The inoculated plates were incubated at 37°C for 18 to 24 h. The antimicrobial activity of each solvent was expressed and measured in terms of the mean diameter of the zone of inhibition (in mm) produced by each solvent at the end of the incubation period. ANOVA was used for intergroup comparison. The P-value of <0.05 was considered significant.
The mean zone of inhibition for was 24.00 ± 1.21 for eucalyptus oil, 16.30 ± 0.92 for chloroform, 26.50 ± 1.24 for turpentine oil, 13.70 ± 1.26 for xylene, and 19.80 ± 1.32 for orange oil. The difference between the groups was statistically significant ( < 0.001).
This study demonstrated that the use of turpentine oil during endodontic retreatment significantly reduced the levels of as compared with other solvents.
使用溶剂去除牙本质小管内充填材料的残余物，以使冲洗液能够渗入小管。
我们旨在确定各种牙胶（GP）溶剂对粪肠球菌的抗菌作用。
采用纸片扩散法测量抑菌圈进行一项研究。该研究使用的受试微生物为粪肠球菌，溶剂分为五组：桉叶油、氯仿、松节油、二甲苯和橙油。将约500μL的悬浮液铺展在琼脂平板上，将空的无菌纸片浸渍10μL纯精油（EOs）。接种后的平板在37°C下孵育18至24小时。每种溶剂的抗菌活性通过孵育期结束时每种溶剂产生的抑菌圈平均直径（以毫米为单位）来表示和测量。采用方差分析进行组间比较。P值<0.05被认为具有统计学意义。
桉叶油对粪肠球菌的平均抑菌圈为24.00±1.21，氯仿为16.30±0.92，松节油为26.50±1.24，二甲苯为13.70±1.26，橙油为19.80±1.32。各组之间的差异具有统计学意义（P<0.001）。
本研究表明，与其他溶剂相比，在根管再治疗期间使用松节油可显著降低粪肠球菌水平。
