Edgar Scott W, Marshall J Gordon, Baumgartner J Craig
Department of Endodontology, Oregon Health Science University, Portland, Oregon 97201, USA.
J Endod. 2006 Dec;32(12):1185-7. doi: 10.1016/j.joen.2006.07.002. Epub 2006 Oct 13.
The purpose of this in vitro study was to evaluate the antimicrobial effectiveness of chloroform on Enterococcus faecalis when used as a gutta-percha solvent during endodontic retreatment. Bilaterally matched human teeth were instrumented, infected with E. faecalis, and obturated. The gutta-percha was then removed using either chloroform or saline. Bacterial samples were collected after gutta-percha removal and following additional apical enlargement. A significant difference was seen (p<0.05) between the number of colony forming units (CFU) of E. faecalis for teeth retreated using chloroform (mean 21+56 CFU/ml) versus saline (mean 280+480 CFU/ml). Negative cultures were obtained in 11 of 17 chloroform samples and none of the saline samples. Samples taken after apical enlargement two sizes larger than the original master apical file showed a significant difference (p<0.05) between teeth retreated using chloroform versus saline. Negative cultures were seen in 9 of 17 chloroform samples and 1 of 17 saline samples. This study demonstrated that the use of chloroform during endodontic retreatment significantly reduced intracanal levels of cultivatable E. faecalis.
本体外研究的目的是评估在根管再治疗期间，当氯仿用作牙胶溶剂时，其对粪肠球菌的抗菌效果。选取双侧匹配的人牙进行预备，感染粪肠球菌，然后进行充填。之后使用氯仿或生理盐水去除牙胶。在去除牙胶后以及进行额外的根尖扩大后采集细菌样本。使用氯仿再治疗的牙齿（平均21 + 56 CFU/ml）与使用生理盐水再治疗的牙齿（平均280 + 480 CFU/ml）相比，粪肠球菌的菌落形成单位（CFU）数量存在显著差异（p<0.05）。17个氯仿样本中有11个培养结果为阴性，而生理盐水样本均为阳性。与原始主尖锉相比，根尖扩大两个号后采集的样本显示，使用氯仿再治疗的牙齿与使用生理盐水再治疗的牙齿之间存在显著差异（p<0.05）。17个氯仿样本中有9个培养结果为阴性，17个生理盐水样本中有1个培养结果为阴性。本研究表明，在根管再治疗期间使用氯仿可显著降低根管内可培养的粪肠球菌水平。
