Stereoselective determination of L-amino acids using column liquid chromatography with an enzymatic solid-phase reactor and chemiluminescence detection.

A stereoselective post-column reaction detection system for eight L-amino acids that makes use of a reactor packed with immobilized L-amino acid oxidase is described. The combination of the selectivity of the enzyme and the selectivity of the peroxyoxalate chemiluminescence detection used provides an extremely selective detection system. The detection system gives linear responses over two orders of magnitude and detection limits at the 0.35.10(-6)-3.0.10(-6) M level. The method was used for the determination of selected L-amino acids in urine and beer.