Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
J Chromatogr A. 2021 Jul 19;1649:462224. doi: 10.1016/j.chroma.2021.462224. Epub 2021 May 7.
For the first time, stepwise pH gradient thin-layer chromatograms of biologically active substances with controlled developing solvent velocity are presented and described in the paper. Change in buffer pH of the mobile phase solution influences retardation, selectivity, and shape of the separated substances' spots. The conducted research has confirmed that the mobile phase's pH gradient could be an essential factor to optimize the conditions of the separation of substances in reversed-phase high-performance thin-layer chromatography. The reproducibility of the gradient retardation factor values of separated substance zones is satisfactory.
本文首次提出并描述了采用分步 pH 梯度和控制展开溶剂速度的生物活性物质薄层层析图谱。流动相溶液缓冲液 pH 值的变化会影响被分离物质斑点的展滞、选择性和形状。研究证实，流动相的 pH 梯度可以成为优化反相高效薄层色谱中物质分离条件的重要因素。分离物质区带的梯度展滞因子值的重现性令人满意。
