Folcik Alexandra M, Klemashevich Cory, Pillai Suresh D
Interdisciplinary Graduate Program in Toxicology, Texas A&M University, College Station, TX, USA.
National Center for Electron Beam Research, An IAEA Collaborating Centre for Electron Beam Technology, Texas A&M University, College Station, TX, USA.
Radiat Phys Chem Oxf Engl 1993. 2021 Sep;186. doi: 10.1016/j.radphyschem.2021.109534. Epub 2021 Apr 28.
Harmful cyanobacterial blooms (cyanoHABs) pose threats to human and animal health due to the production of harmful cyanotoxins. is a common cyanobacterium associated with these blooms and is responsible for producing the potent cyclic hepatotoxin microcystin-LR (MC-LR). Concerns over the public health implications of these toxins in water supplies have increased due to rising occurrence of these blooms. High energy electron beam (eBeam) irradiation technology presents a promising strategy for the mitigation of both cyanobacterial cells and cyanotoxins within the water treatment process. However, it is imperative that both cellular and chemical responses to eBeam irradiation are understood to ensure efficient treatment. We sought to investigate the effect of eBeam irradiation on cells and MC-LR degradation. Results indicate that doses as low as 2 kGy are lethal to cells and induce cell lysis. Even lower doses are required for degradation of the parent MC-LR toxin. However, it was observed that there is a delay in cell lysis after irradiation where cells may still be metabolically active and able to synthesize microcystin. These results suggest that eBeam may be suitable for cyanoHAB mitigation in water treatment if employed following cell lysis.
有害蓝藻水华（cyanoHABs）由于产生有害蓝藻毒素而对人类和动物健康构成威胁。是与这些水华相关的常见蓝藻，负责产生强效环状肝毒素微囊藻毒素-LR（MC-LR）。由于这些水华的发生率不断上升，人们对这些毒素对供水的公共卫生影响的担忧也与日俱增。高能电子束（eBeam）辐照技术为在水处理过程中减轻蓝藻细胞和蓝藻毒素提供了一种有前景的策略。然而，必须了解细胞和化学对电子束辐照的反应，以确保有效处理。我们试图研究电子束辐照对细胞和MC-LR降解的影响。结果表明，低至2 kGy的剂量对细胞具有致死性并诱导细胞裂解。降解母体MC-LR毒素所需的剂量甚至更低。然而，观察到辐照后细胞裂解存在延迟，此时细胞可能仍具有代谢活性并能够合成微囊藻毒素。这些结果表明，如果在细胞裂解后使用，电子束可能适用于水处理中的cyanoHAB缓解。
