Nyyssönen K, Parviainen M T, Penttilä I M
Department of Clinical Chemistry, Kuopio University Central Hospital, Finland.
J Clin Chem Clin Biochem. 1988 Apr;26(4):219-22. doi: 10.1515/cclm.1988.26.4.219.
Plasma haemoglobin was assayed with the non-carcinogenic reagent phenothiazine. This method is sensitive and allows the measurement of plasma haemoglobin concentrations in the range 4-500 mg/l with a within-run CV of 2.1%, and a between-run CV of 4.3%. A spectrophotometric scanning method (x) based on the determination of haemoglobin as haemiglobin cyanide using the Soret band at 419 nm correlated well with the phenothiazine method (y): y = 1.07x + 15.8, r = 0.995, n = 31. It was found that the absorbances in the phenothiazine method were markedly dependent on the concentration of phosphoric acid.
采用非致癌试剂吩噻嗪测定血浆血红蛋白。该方法灵敏度高，可测量浓度范围为4 - 500 mg/l的血浆血红蛋白，批内变异系数为2.1%，批间变异系数为4.3%。一种基于在419 nm处使用索雷特带将血红蛋白测定为氰化高铁血红蛋白的分光光度扫描法(x)与吩噻嗪法(y)相关性良好：y = 1.07x + 15.8，r = 0.995，n = 31。研究发现，吩噻嗪法中的吸光度明显取决于磷酸浓度。
