马铃薯X病毒的完整核苷酸序列及其在氨基酸水平上与各种正链RNA病毒的同源性。

The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses.

作者信息

Huisman M J, Linthorst H J, Bol J F, Cornelissen J C

机构信息

MOGEN International N.V., Leiden, The Netherlands.

出版信息

J Gen Virol. 1988 Aug;69 ( Pt 8):1789-98. doi: 10.1099/0022-1317-69-8-1789.

DOI:10.1099/0022-1317-69-8-1789

PMID:3404114

Abstract

Double-stranded cDNA of potato virus X (PVX) genomic RNA has been cloned and sequenced. The sequence [6435 nucleotides excluding the poly(A) tract] revealed five open reading frames (ORFs) which were numbered one to five starting at the 5' terminus of the RNA. They encoded proteins of Mr 165588 (166K), 24622 (25K), 12324 (12K), 7595 (8K) and 25080 (coat protein), respectively. ORFs 1 and 2 were inphase coding regions. The ORF 1 product contained domains of homology with the tobacco mosaic virus 126K and 183K products. The ORF 2 and 3 products showed homologies with the barley stripe mosaic virus 58K and 14K proteins, the beet necrotic yellow vein virus 42K and 13K products and the white clover mosaic virus 26K and 13K products, respectively. The significance of these homologies with respect to putative functions of the PVX-encoded proteins are discussed.

摘要

马铃薯X病毒（PVX）基因组RNA的双链cDNA已被克隆并测序。该序列（不包括聚腺苷酸尾，共6435个核苷酸）显示有五个开放阅读框（ORF），从RNA的5'末端开始依次编号为1至5。它们分别编码分子量为165588（166K）、24622（25K）、12324（12K）、7595（8K）和25080（外壳蛋白）的蛋白质。开放阅读框1和2是同相编码区。开放阅读框1的产物包含与烟草花叶病毒126K和183K产物同源的结构域。开放阅读框2和3的产物分别与大麦条纹花叶病毒58K和14K蛋白、甜菜坏死黄脉病毒42K和13K产物以及白三叶草花叶病毒26K和13K产物具有同源性。文中讨论了这些同源性对于PVX编码蛋白假定功能的意义。