Sit T L, White K A, Holy S, Padmanabhan U, Eweida M, Hiebert M, Mackie G A, AbouHaidar M G
Department of Botany, University of Toronto, Ontario, Canada.
J Gen Virol. 1990 Sep;71 ( Pt 9):1913-20. doi: 10.1099/0022-1317-71-9-1913.
The entire genomic RNA of clover yellow mosaic virus was sequenced from cDNA clones and run-off cDNA transcripts. The genomic RNA is 7015 nucleotides in length [excluding a 3' poly(A) tail], with six open reading frames (ORFs) greater than 150 nucleotides in length. The first five ORFs encode proteins of Mr 191K, 26K, 12K, 6.5K and 28K, respectively. The sixth ORF lies completely within ORF1 and codes for a protein of Mr 14K. The capsid protein coding region (Mr 23K) is found within ORF5 which encodes the Mr 28K protein. Proteins encoded by ORFs 1 to 3 and ORF5 show strong homology with proteins of other potexviruses, especially papaya mosaic virus.
从 cDNA 克隆和 runoff cDNA 转录本对三叶草黄花叶病毒的全基因组 RNA 进行了测序。基因组 RNA 长度为 7015 个核苷酸[不包括 3' 聚(A)尾]，有六个长度大于 150 个核苷酸的开放阅读框(ORF)。前五个 ORF 分别编码 Mr 191K、26K、12K、6.5K 和 28K 的蛋白质。第六个 ORF 完全位于 ORF1 内，编码 Mr 14K 的蛋白质。衣壳蛋白编码区(Mr 23K)位于 ORF5 内，ORF5 编码 Mr 28K 蛋白质。ORF1 至 3 和 ORF5 编码的蛋白质与其他马铃薯 Y 病毒属病毒的蛋白质具有很强的同源性，尤其是木瓜花叶病毒。
