Forster R L, Bevan M W, Harbison S A, Gardner R C
Plant Diseases Division, DSIR, Auckland, New Zealand.
Nucleic Acids Res. 1988 Jan 11;16(1):291-303. doi: 10.1093/nar/16.1.291.
The complete nucleotide sequence (5845 nucleotides) of the genomic RNA of the potexvirus white clover mosaic virus (WC1MV) has been determined from a set of overlapping cDNA clones. Forty of the most 5'-terminal nucleotides of WC1MV showed homology to the 5' sequences of other potexviruses. The genome contained five open reading frames which coded for proteins of Mr 147, 417, Mr 26,356, Mr 12,989, Mr 7,219 and Mr 20,684 (the coat protein). The Mr 147,417 protein had domains of amino acid sequence homology with putative polymerases of other RNA viruses. The Mr 26,356 and Mr 12,989 proteins had homology with proteins of the hordeivirus barley stripe mosaic virus RNA beta and the furovirus beet necrotic yellow vein virus (BNYVV) RNA-2. A portion of the Mr 26,356 protein was also conserved in the cylindrical inclusion proteins of two potyviruses. The Mr 7,219 protein had homology with the 25K putative fungal transmission factor of BNYVV RNA-3.
已从一组重叠的 cDNA 克隆中确定了马铃薯 X 病毒属白三叶草花叶病毒（WC1MV）基因组 RNA 的完整核苷酸序列（5845 个核苷酸）。WC1MV 最 5' 端的 40 个核苷酸与其他马铃薯 X 病毒属病毒的 5' 序列具有同源性。该基因组包含五个开放阅读框，它们编码的蛋白质分子量分别为 147、417、26356、12989、7219 和 20684（外壳蛋白）。分子量为 147417 的蛋白质具有与其他 RNA 病毒推定聚合酶的氨基酸序列同源结构域。分子量为 26356 和 12989 的蛋白质与大麦病毒属大麦条纹花叶病毒 RNAβ 和真菌传杆状病毒属甜菜坏死黄脉病毒（BNYVV）RNA - 2 的蛋白质具有同源性。分子量为 26356 的蛋白质的一部分在两种马铃薯 Y 病毒的柱状内含体蛋白中也保守。分子量为 7219 的蛋白质与 BNYVV RNA - 3 的 25K 推定真菌传播因子具有同源性。
