Alses Mohammad, Alzeer Samar
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Damascus, Damascus, Syria.
Heliyon. 2021 May 20;7(5):e07056. doi: 10.1016/j.heliyon.2021.e07056. eCollection 2021 May.
Gasoline is a blend of organic compounds used in internal combustion engines. gasoline station attendants (GSA) are exposed to gasoline vapours, which promotes oxidative stress through the production of ROS, which can damage biological structures with the formation of new metabolites which can be used as markers of oxidant/antioxidant imbalance. This is a comparative cross-sectional study. This study aims to evaluate some biological parameters as indicators of toxicity as a result of exposure to gasoline in workers at gas stations in Damascus.
Blood samples were collected from GSA (n = 30) and non-exposed (NE) (n = 30) with no history of occupational exposure, and the following markers of oxidative stress were analysed: malondialdehyde (MDA), advanced oxidation protein products (AOPP), catalase activity (CAT), CBC, ALT and AST.
We have found that the levels of MDA, AOPP, CAT, RBC and Hgb in GSA were significantly higher than NE (p = 0.000, p = 0.02, p = 0.002, p = 0.018 and p = 0.015 respectively). On the other hand, there were no statistically significant (p > 0.05) in HCT, MCV, WBC, PLT, ALT and AST between the two groups. In the GSA group, there was no effect of the smoking habit and the number of years of work on biological parameters, but alcohol consumption habit had a clear effect on increasing both levels of MDA and CAT ( = 0.021 and = 0.036 respectively), in comparison to the non-consumers of the alcohol group. The results from our study showed that chronic gasoline exposure may result in long-lasting oxidative stress, as demonstrated by the presence of statistically significant correlations between gasoline exposure and levels of biomarkers (MDA, AOPPs, Catalase activity).
the early identification of these biomarkers can be very useful to promote programs on health protection and prevention for those populations more susceptible to the adverse effects of gasoline exposure.
汽油是用于内燃机的有机化合物混合物。加油站工作人员（GSA）会接触汽油蒸气，汽油蒸气通过产生活性氧（ROS）促进氧化应激，ROS会形成新的代谢产物，这些代谢产物会损害生物结构，可作为氧化/抗氧化失衡的标志物。这是一项比较性横断面研究。本研究旨在评估大马士革加油站工作人员因接触汽油而产生毒性的一些生物学参数指标。
采集了30名加油站工作人员（GSA）和30名无职业接触史的非接触者（NE）的血样，分析了以下氧化应激标志物：丙二醛（MDA）、晚期氧化蛋白产物（AOPP）、过氧化氢酶活性（CAT）、全血细胞计数（CBC）、谷丙转氨酶（ALT）和谷草转氨酶（AST）。
我们发现，加油站工作人员组中MDA、AOPP、CAT、红细胞（RBC）和血红蛋白（Hgb）水平显著高于非接触者组（分别为p = 0.000、p = 0.02、p = 0.002、p = 0.018和p = 0.015）。另一方面，两组间血细胞比容（HCT）、平均红细胞体积（MCV）、白细胞（WBC）、血小板（PLT）、ALT和AST无统计学显著差异（p > 0.05）。在加油站工作人员组中，吸烟习惯和工作年限对生物学参数无影响，但与不饮酒组相比，饮酒习惯对MDA和CAT水平升高有明显影响（分别为p = 0.021和p = 0.036）。我们的研究结果表明，长期接触汽油可能导致持久的氧化应激，汽油接触与生物标志物（MDA、AOPPs、过氧化氢酶活性）水平之间存在统计学显著相关性即证明了这一点。
早期识别这些生物标志物对于为那些更容易受到汽油接触不良影响的人群推广健康保护和预防计划非常有用。
