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玫瑰茄包被银纳米芯片对伴放线聚集杆菌的抗菌活性

Antimicrobial Activity of Roselle-capped​ Silver​ Nanochip on Aggregatibacter actinomycetemcomitans.

作者信息

Wacharanad Sirorat, Thatree Puncharee, Yiemwattana Punchaya, Paoprajak Penpitcha, Ngamsangiam Pimchanok, Valyanont Menisa, Yiemwattana Ichaya

机构信息

Department of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand.

Secondary Demonstration School, Naresuan University, Phitsanulok, Thailand.

出版信息

Eur J Dent. 2021 Jul;15(3):574-578. doi: 10.1055/s-0041-1725574. Epub 2021 May 26.

DOI:10.1055/s-0041-1725574
PMID:34041731
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8382462/
Abstract

OBJECTIVES

This article aimed to study the effects of the​ roselle-capped​ silver​ nanochip​ ​(SNP-Ro​ chip)​ against , and the toxicity of this film on fibroblast cells to develop this SNP-Ro chip into a local chemical for the treatment of periodontitis in the future.

MATERIALS AND METHODS

Using a microwave-assisted synthesis method, silver​ nanoparticles (SNPs) were prepared from a silver nitrate solution and roselle extract as a reducing and capping agent. Then, SNP-Ro chips were fabricated by mixing a solution of SNP-Ro with alginate gel. The antimicrobial effect of the synthesized SNP-Ro chips was performed by the disc diffusion technique and time kill assay. The cytotoxic effect was also determined by the MTS assay.

STATISTICAL ANALYSIS

One-way analysis of variance (ANOVA) and Scheffe's method were used to analyze the data for this experiment.

RESULTS

All three ratios of the SNP-Ro chip produced inhibition zones ranging between 18.75 ± 2.08 and 19.03 ± 2.25 mm. In studying the killing time, the three groups of the SNP-Ro chips completely eradicated within 180 minutes. The percentage of the viable SNP-Ro chip-treated human gingival fibroblasts (HGFs) were significantly increased when compared with the alginate chip-treated cells ( < 0.05).

CONCLUSION

This study developed a new method for the deposition of SNPs in alginate gel to make a thin small chip for the sustained release of the SNPs in a periodontal lesion. Therefore, the SNP-Ro chip has the potential to be developed as an adjunctive locally delivered antimicrobial agent in periodontal therapy.

摘要

目的

本文旨在研究玫瑰茄包覆银纳米芯片（SNP-Ro芯片）的抗菌效果及其对成纤维细胞的毒性，以便未来将这种SNP-Ro芯片开发成一种治疗牙周炎的局部用药。

材料与方法

采用微波辅助合成法，以硝酸银溶液和玫瑰茄提取物作为还原剂和包覆剂制备银纳米颗粒（SNPs）。然后，将SNP-Ro溶液与海藻酸钠凝胶混合制成SNP-Ro芯片。通过纸片扩散法和时间杀菌试验检测合成的SNP-Ro芯片的抗菌效果。细胞毒性也通过MTS试验测定。

统计分析

采用单因素方差分析（ANOVA）和谢弗法对本实验数据进行分析。

结果

SNP-Ro芯片的三种比例均产生了18.75±2.08至19.03±2.25毫米的抑菌圈。在研究杀菌时间时，三组SNP-Ro芯片在180分钟内完全消除。与海藻酸钠芯片处理的细胞相比，经SNP-Ro芯片处理的人牙龈成纤维细胞（HGFs）的存活百分比显著增加（P<0.05）。

结论

本研究开发了一种在海藻酸钠凝胶中沉积SNPs的新方法，制成了一种薄的小芯片，用于在牙周病变中持续释放SNPs。因此，SNP-Ro芯片有潜力被开发成为牙周治疗中辅助局部递送的抗菌剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/3b769327798f/10-1055-s-0041-1725574_10_1061_05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/d8756c37b0ed/10-1055-s-0041-1725574_10_1061_01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/72ba37a29d8e/10-1055-s-0041-1725574_10_1061_02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/271811ec7b12/10-1055-s-0041-1725574_10_1061_03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/c0a838fce459/10-1055-s-0041-1725574_10_1061_04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/3b769327798f/10-1055-s-0041-1725574_10_1061_05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/d8756c37b0ed/10-1055-s-0041-1725574_10_1061_01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/72ba37a29d8e/10-1055-s-0041-1725574_10_1061_02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/271811ec7b12/10-1055-s-0041-1725574_10_1061_03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/c0a838fce459/10-1055-s-0041-1725574_10_1061_04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2439/8382462/3b769327798f/10-1055-s-0041-1725574_10_1061_05.jpg

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