Reise Markus, Gottschaldt Michael, Matz Carina, Völpel Andrea, Jandt Klaus D, Schubert Ulrich S, Sigusch Bernd W
Department of Conservative Dentistry and Periodontology, Jena University Hospital, An der Alten Post 4, 07743, Jena, Germany.
Laboratory of Organic and Macromolecular Chemistry, Friedrich Schiller University Jena, Humboldtstr. 10, 07743, Jena, Germany.
BMC Oral Health. 2016 Mar 23;16:42. doi: 10.1186/s12903-016-0201-4.
It was the aim of this study to evaluate the antibacterial impact of two silver(I) carbohydrate complexes with tripodal thioglycosides, namely tris[2-(β-D-thio-glucopyranosyl)ethyl]-amine-silver(I)-nitrate (3) and tris[2-(α-D-thio-manno-pyranosyl)ethyl]-amine-silver(I)-nitrate (4), on five oral pathogenic bacterial strains. Furthermore, cytocompatibility was tested using human gingival fibroblasts (HGF).
Minimum inhibitory concentrations (MIC) were determined on five oral pathogenic bacterial strains by using the broth microdilution method: Fusobacterium nucleatum (ATCC 10953), Aggregatibacter actinomycetemcomitans (ATCC 33384), Porphyromonas gingivalis (ATCC 33277), Streptococcus mutans (ATCC 25175) and Enterococcus faecalis (DSMZ 20376). Furthermore, antimicrobial efficiency was tested using agar diffusion assays. To evaluate cytocompatibility, human gingival fibroblasts (HGFs) were exposed to AgNO3 and complex 3 followed by a live/dead staining.
MIC of the silver(I) complexes ranged between 0.625 and 5.0 mmol/L. The silver complexes 3 and 4 showed higher antibacterial efficiency against all tested species than AgNO3. Antibacterial efficiency of complexes 3 and 4 on F. nucleatum (≥18 mm) and A. actinomycetemcomitans (≥23 mm) was more pronounced than against P. gingivalis (≥15 mm). Complex 3 (20 mM) induced the largest inhibition zones (30 to 31 mm) on Gram-negative strains. For Gram-positive strains, the largest inhibition zones were achieved by complex 3 (20 mM/S. mutans: 28 mm, E. faecalis: 18 mm). Complex 3 had a lower cytotoxic impact on HGFs compared to AgNO3 by the power of ten.
The findings suggest that silver(I) carbohydrate complexes 3 and 4 might function as novel antimicrobial agents for the treatment of periodontal, carious or endodontic diseases.
本研究旨在评估两种含三脚架硫代糖苷的一价银碳水化合物配合物，即三[2-(β-D-硫代吡喃葡萄糖基)乙基]胺-硝酸银(I)(3)和三[2-(α-D-硫代甘露糖基)乙基]胺-硝酸银(I)(4)对五种口腔致病细菌菌株的抗菌作用。此外，使用人牙龈成纤维细胞(HGF)测试了细胞相容性。
采用肉汤微量稀释法测定五种口腔致病细菌菌株的最低抑菌浓度(MIC)：具核梭杆菌(ATCC 10953)、伴放线聚集杆菌(ATCC 33384)、牙龈卟啉单胞菌(ATCC 33277)、变形链球菌(ATCC 25175)和粪肠球菌(DSMZ 20376)。此外，使用琼脂扩散试验测试抗菌效率。为了评估细胞相容性，将人牙龈成纤维细胞(HGFs)暴露于硝酸银和配合物3中，然后进行活/死染色。
一价银配合物的MIC范围在0.625至5.0 mmol/L之间。银配合物3和4对所有测试菌种的抗菌效率均高于硝酸银。配合物3和4对具核梭杆菌(≥18 mm)和伴放线聚集杆菌(≥23 mm)的抗菌效率比对牙龈卟啉单胞菌(≥15 mm)更显著。配合物3(20 mM)对革兰氏阴性菌株诱导的抑菌圈最大(30至31 mm)。对于革兰氏阳性菌株，配合物3(20 mM)产生的抑菌圈最大(变形链球菌：28 mm，粪肠球菌：18 mm)。与硝酸银相比，配合物3对HGFs的细胞毒性影响低10倍。
研究结果表明，一价银碳水化合物配合物3和4可能作为治疗牙周病、龋齿或牙髓病的新型抗菌剂。
