Hacene Youcef Chakib, Loiseau Alexis, Maio Vanessa Dos Passos, Grenier Philippe, Boisselier Elodie, Bertrand Nicolas
Faculty of Pharmacy, CHU de Quebec Research Center, Université Laval, CHUL, 2705 Laurier Blvd, Québec G1 V 4G2, Canada.
Faculty of Medicine, Department of Ophthalmology, CHU de Québec Research Center, Université Laval, Hôpital du Saint-Sacrement, CUO-Recherche, 1050, chemin Sainte-Foy, Québec, G1S 4L8, Canada.
Nano Lett. 2021 Jun 9;21(11):4530-4538. doi: 10.1021/acs.nanolett.0c05056. Epub 2021 May 27.
Polyethylene glycol (PEG) is considered the gold standard to prepare long circulating nanoparticles. The hydrophilic layer that sterically protects PEGylated nanomedicines also impedes their separation from biological media. In this study, we describe an immunoprecipitation method using AntiPEG antibodies cross-linked to magnetic beads to extract three types of radiolabeled PEGylated systems: polymeric nanoparticles, liposomes, and therapeutic proteins. The potential of the method is emphasized by isolating these systems after in vivo administration and ex vivo incubation in human biological fluids. Immunoprecipitation also allows a unique perspective on the size distribution of nanoparticles in the bloodstream after intravenous and intraperitoneal administrations. Further, we highlight the potential of the approach to inform on nanomaterial-associated drug in plasma as well as help characterize the protein corona. Altogether, we believe this method answers an unmet need in nanomedicine research and will contribute a fresh perspective on the interactions of nanomedicines with biological systems.
聚乙二醇（PEG）被认为是制备长循环纳米颗粒的金标准。在空间上保护聚乙二醇化纳米药物的亲水层也阻碍了它们与生物介质的分离。在本研究中，我们描述了一种免疫沉淀方法，该方法使用与磁珠交联的抗PEG抗体来提取三种类型的放射性标记聚乙二醇化系统：聚合物纳米颗粒、脂质体和治疗性蛋白质。通过在体内给药和在人类生物流体中进行体外孵育后分离这些系统，强调了该方法的潜力。免疫沉淀还为静脉内和腹腔内给药后血液中纳米颗粒的大小分布提供了独特的视角。此外，我们强调了该方法在了解血浆中纳米材料相关药物以及帮助表征蛋白质冠方面的潜力。总之，我们相信这种方法满足了纳米医学研究中未满足的需求，并将为纳米药物与生物系统的相互作用提供新的视角。
