Hsiao Hui-Yi, Lai Chao-Yi, Liu Jia-Wei, Yu Yuan-Yuan, Chang Frank Chun-Shin, Huang Jung-Ju
Center for Tissue Engineering, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Division of Craniofacial Surgery, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Aesthet Surg J. 2021 Sep 14;41(10):NP1323-NP1336. doi: 10.1093/asj/sjab231.
Recently, there has been increasing research interest in identifying the effect of liposuction procedures on fat graft survival in order to clarify whether different harvest techniques affect the quality of fat grafts.
The aim of this study was to investigate the effect of 2 liposuction methods on the survival and regeneration potential of grafted fat tissue. The proliferation and differentiation potentials of adipose-derived stem cells (ASCs) isolated by both methods was also investigated.
Fat grafts were collected from patients who underwent liposuction procedures by 2 different methods: traditional suction-assisted liposuction (TSAL) and vibration amplification of sound energy at resonance (VASER). One portion of the lipoaspirates was implanted into the subcutaneous layer of nu mice for 4 and 12 weeks. ASCs were isolated from the other portion of the lipoaspirate and subjected to proliferation and differentiation assays.
Although in vivo fat grafting presented similar adipose tissue survival for the 2 different liposuction methods, more angiogenesis and less fibrosis was observed in the VASER group based on histologic evaluation. Furthermore, VASER-derived ASCs presented better quality in terms of cell differentiation capacity.
The in vivo study confirmed better graft angiogenesis with less inflammation, apoptosis, and scar formation in the VASER group. ASCs harvested with VASER exhibited increased differentiation capacity compared with those obtained by TSAL, and represent an excellent source for fat grafting and regenerative medicine.
最近，为了阐明不同的采集技术是否会影响脂肪移植的质量，关于确定抽脂手术对脂肪移植存活影响的研究兴趣日益浓厚。
本研究的目的是调查两种抽脂方法对移植脂肪组织存活和再生潜力的影响。还研究了通过这两种方法分离的脂肪来源干细胞（ASC）的增殖和分化潜力。
通过两种不同方法对接受抽脂手术的患者采集脂肪移植：传统的抽吸辅助抽脂（TSAL）和共振声能振动放大抽脂（VASER）。将一部分抽脂物植入裸鼠皮下层4周和12周。从抽脂物的另一部分分离出ASC，并进行增殖和分化分析。
尽管在体内脂肪移植中，两种不同抽脂方法的脂肪组织存活率相似，但基于组织学评估，VASER组观察到更多的血管生成和更少的纤维化。此外，VASER来源的ASC在细胞分化能力方面表现出更好的质量。
体内研究证实，VASER组移植的血管生成更好，炎症、细胞凋亡和瘢痕形成更少。与TSAL获得的ASC相比，VASER采集的ASC表现出更高的分化能力，是脂肪移植和再生医学的优质来源。
