Molecular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan.
Department of Molecular Brain Science, Graduate School of Life Sciences, Kumamoto University, Kumamoto, Japan.
Neuropsychopharmacol Rep. 2021 Sep;41(3):416-421. doi: 10.1002/npr2.12178. Epub 2021 May 27.
Genetic and environmental factors interact with each other to influence the risk of various psychiatric diseases; however, the intensity and nature of their interactions remain to be elucidated. We established a maternal infection model using polyinosinic-polycytidylic acid (Poly(I:C)) to determine the relationship between the maternal breeding environment and behavioral changes in the offspring. We purchased pregnant C57BL/6J mice from three breeders and administered Poly(I:C) (2 mg/kg) intravenously in their tail vein on gestation day 15. The offspring were raised to 8-12 weeks old and subjected to the acoustic startle tests to compare their startle response intensity, prepulse inhibition levels, and degree of the adaptation of the startle response. No statistical interaction between Poly(I:C) administration and sex was observed for prepulse inhibition; thus, male and female mice were analyzed together. There was a statistical interaction between the breeder origin of offspring and prepulse inhibition; the Poly(I:C) challenge significantly decreased prepulse inhibition levels of the offspring born to the pregnant dams from Breeder A but not those from the other breeders. However, we failed to detect significant inter-breeder differences in Poly(I:C) effects on startle response and on startle adaptation with the given number of mice examined. The rearing environment of mouse dams has a prominent effect on the Poly(I:C)-induced prepulse inhibition deficits in this maternal immune activation model.
遗传和环境因素相互作用，影响各种精神疾病的风险；然而，它们相互作用的强度和性质仍有待阐明。我们建立了一个母体感染模型，使用聚肌胞苷酸（Poly(I:C)) 来确定母体繁殖环境与后代行为变化之间的关系。我们从三个饲养员处购买了怀孕的 C57BL/6J 小鼠，并在妊娠第 15 天通过尾静脉给它们注射 2mg/kg 的 Poly(I:C)。将后代饲养至 8-12 周龄，并进行听觉惊跳测试，以比较它们的惊跳反应强度、前脉冲抑制水平和惊跳反应适应程度。前脉冲抑制没有观察到 Poly(I:C)给药和性别之间的统计学相互作用；因此，雄性和雌性小鼠一起进行分析。后代的饲养员来源与前脉冲抑制之间存在统计学相互作用；母体免疫激活模型中，聚肌胞苷酸挑战显著降低了来自饲养员 A 的孕鼠所生后代的前脉冲抑制水平，但对其他饲养员的后代没有影响。然而，在用所检查的老鼠数量进行检测时，我们未能发现 Poly(I:C)对惊跳反应和惊跳适应的影响在饲养员之间存在显著差异。鼠母的饲养环境对母体免疫激活模型中聚肌胞苷酸诱导的前脉冲抑制缺陷有显著影响。
