College of Marine Science, Ningbo University, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo, China; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China.
College of Marine Science, Ningbo University, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo, China; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China.
Tissue Cell. 2021 Oct;72:101558. doi: 10.1016/j.tice.2021.101558. Epub 2021 May 21.
Mitochondria play crucial roles during oocyte development. In this study, we have investigated mitochondrial morphology, mtDNA, Ca-ATP enzyme activity, and mitochondrial fission factor (mff) expression levels during oogenesis of the silver pomfret Pampus argenteus. The mtDNA increased with oocyte development, and mitochondrial morphology and distribution were stage-specific. In the perinucleolar oocytes, oval mitochondria were dispersed in the cytoplasm. In previtellogenic oocytes, mitochondria massively increased and aggregated, forming mitochondrial clouds. At the same time, two morphologically different types of mitochondria had been distinguished, one of which was elongated with well-developed cristae, and the other was round with distorted and fused cristae. During vitellogenesis, the increases in mitochondria with well-developed cristae and in Ca-ATPase enzymatic activity were accompanied by an accumulation of yolk substance, suggesting the possible participation of mitochondria in the formation of vitellogenesis. Furthermore, we examined the cDNA of mff its transcript levels in relation to oocyte development. The transcript levels of mff were high in the perinucleolar stage, increasing to the highest level at the previtellogenic stage. Immunocytochemistry showed that MFF was detected in the cytoplasm of previtellogenic and midvitellogenic oocytes. We speculated that the mff-mediated mitochondrial fission may play a crucial role in oocyte development, especially in vitellogenesis.
线粒体在卵母细胞发育过程中发挥着至关重要的作用。在本研究中，我们研究了银鲳 Pampus argenteus 卵母细胞发生过程中线粒体形态、mtDNA、Ca-ATP 酶活性和线粒体分裂因子（mff）的表达水平。mtDNA 随着卵母细胞的发育而增加，线粒体的形态和分布具有阶段特异性。在核周卵母细胞中，椭圆形线粒体分散在细胞质中。在卵黄发生前期，线粒体大量增加并聚集，形成线粒体云。同时，已经区分出两种形态不同的线粒体，一种是长形的，嵴发育良好，另一种是圆形的，嵴扭曲和融合。在卵黄发生过程中，伴随着卵黄物质的积累，具有良好嵴的线粒体和 Ca-ATP 酶活性的增加，表明线粒体可能参与了卵黄发生的形成。此外，我们还研究了 mff 的 cDNA 及其与卵母细胞发育相关的转录水平。mff 的转录水平在核周期较高，在卵黄发生前期达到最高水平。免疫细胞化学显示 MFF 存在于卵黄发生前期和中期卵母细胞的细胞质中。我们推测 mff 介导的线粒体分裂可能在卵母细胞发育，特别是在卵黄发生中起着关键作用。
