Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, Doha 2713, Qatar; Biomedical Research Center (BRC), Qatar University, Doha 2713, Qatar.
Pushpagiri Research Centre, Pushpagiri Institute of Medical Sciences & Research, Tiruvalla 689101, Kerala, India.
Biomed Pharmacother. 2021 Aug;140:111747. doi: 10.1016/j.biopha.2021.111747. Epub 2021 May 25.
Management of non-healing and slow to heal diabetic wounds is a major concern in healthcare across the world. Numerous techniques have been investigated to solve the issue of delayed wound healing, though, mostly unable to promote complete healing of diabetic wounds due to the lack of proper cell proliferation, poor cell-cell communication, and higher chances of wound infections. These challenges can be minimized by using hydrogel based wound healing patches loaded with bioactive agents. Gelatin methacrylate (GelMA) has been proven to be a highly cell friendly, cell adhesive, and inexpensive biopolymer for various tissue engineering and wound healing applications. In this study, S-Nitroso-N-acetylpenicillamine (SNAP), a nitric oxide (NO) donor, was incorporated in a highly porous GelMA hydrogel patch to improve cell proliferation, facilitate rapid cell migration, and enhance diabetic wound healing. We adopted a visible light crosslinking method to fabricate this highly porous biodegradable but relatively stable patch. Developed patches were characterized for morphology, NO release, cell proliferation and migration, and diabetic wound healing in a rat model. The obtained results indicate that SNAP loaded visible light crosslinked GelMA hydrogel patches can be highly effective in promoting diabetic wound healing.
管理非愈合和愈合缓慢的糖尿病伤口是全球医疗保健的一个主要关注点。已经研究了许多技术来解决伤口愈合延迟的问题，但由于缺乏适当的细胞增殖、细胞间通讯不良以及更高的伤口感染风险，大多数技术都无法促进糖尿病伤口的完全愈合。通过使用负载生物活性剂的水凝胶基伤口愈合贴片可以最小化这些挑战。明胶甲基丙烯酰胺（GelMA）已被证明是一种非常适合细胞的、具有细胞黏附性的、廉价的生物聚合物，可用于各种组织工程和伤口愈合应用。在这项研究中，S-亚硝基-N-乙酰青霉胺（SNAP），一种一氧化氮（NO）供体，被掺入高度多孔的 GelMA 水凝胶贴片，以提高细胞增殖、促进快速细胞迁移和增强糖尿病伤口愈合。我们采用可见光交联方法来制备这种高度多孔的可生物降解但相对稳定的贴片。对所开发的贴片进行了形态学、NO 释放、细胞增殖和迁移以及糖尿病大鼠模型中的伤口愈合的特性研究。得到的结果表明，负载 SNAP 的可见光交联 GelMA 水凝胶贴片可非常有效地促进糖尿病伤口愈合。
