College of Biology, Hunan University, Changsha, Hunan 410082, People's Republic of China.
State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University, Changsha, Hunan 410082, People's Republic of China.
Biofabrication. 2021 Jul 5;13(3). doi: 10.1088/1758-5090/ac0610.
There is a critical need to developculture systems appropriate for the expansion of adipose tissue, in order to gain new insights into metabolic diseases and to assist in the restoration of tissue defects. Conventional two- or three-dimensional (2D or 3D)models of adipocytes require a combination of supplements to induce adipocyte maturation that greatly increases the cost of large-scale industrial production. In the present study, a microporous, perforated bacterial cellulose (BC)-assisted culture system was developed that promoted the adhesion, proliferation, and adipogenic differentiation of preadipocytes. Additionally, the system maintained the cells as mature unilocular adipocytesin normal cell culture medium in long-term culture. All cells were derived from isolated adipose tissue without the use of expensive enzymes for tissue digestion. In contrast to culture in hard tissue culture plates, preadipocytes in the soft 3D environments formed multidimensional interlaced cell contacts, undergoing significant spontaneous lipid accumulation and could be cultured for up to threemonths in maintenance medium. More importantly, the cultured adipose tissue-derived cell bank created here was able to produce injury repair activators that promoted the proliferation of fibroblasts with little fibrosis and the functional differentiation of myoblasts, displaying the potential for use in adipose reconstruction. Thus, the present study demonstrates the potential of a mechanically flexible BC scaffold to generate volume tunable adipose constructs and provides a low-cost and user-friendly strategy for large-scale industrial production of adipose tissue.
为了深入了解代谢性疾病并协助组织缺陷的修复，急需开发适合脂肪组织扩增的培养体系。传统的二维或三维（2D 或 3D）脂肪细胞模型需要添加多种补充剂来诱导脂肪细胞成熟，这极大地增加了大规模工业化生产的成本。本研究开发了一种多孔、穿孔的细菌纤维素（BC）辅助培养体系，可促进前脂肪细胞的黏附、增殖和脂肪生成分化。此外，该体系可在长期培养中维持细胞成为成熟的单房脂肪细胞，且使用的是普通细胞培养基，无需昂贵的酶进行组织消化。与硬组织培养板相比，软 3D 环境中的前脂肪细胞形成了多维交错的细胞接触，经历显著的自发脂质积累，可在维持培养基中培养长达三个月。更重要的是，我们成功创建了可用于脂肪重建的细胞库，这些细胞库能够产生促进成纤维细胞增殖、少纤维化和肌母细胞功能分化的损伤修复激活剂。因此，本研究证明了机械柔性 BC 支架在生成可调节体积的脂肪组织方面具有潜力，并为大规模工业化生产脂肪组织提供了一种低成本、用户友好的策略。
