鉴定与食管鳞癌患者预后相关的肿瘤浸润淋巴细胞亚群。
Identification of tumor-infiltrating lymphocyte subpopulations correlated with patient prognosis in esophageal squamous cell carcinoma.
机构信息
Department of Thoracic Surgery, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, China.
出版信息
J Int Med Res. 2021 May;49(5):3000605211016206. doi: 10.1177/03000605211016206.
OBJECTIVE
To identify biomarkers related to esophageal squamous cell carcinoma (ESCC) prognosis by analyzing genetic variations and the infiltration levels of tumor-infiltrating lymphocytes (TILs) in patients.
METHODS
The clinical features of 61 patients with ESCC were collected. DNA panel sequencing was performed to screen differentially expressed genes (DEGs). Transcriptome sequencing was performed to identify gene expression profiles, and subsequent enrichment analysis of DEGs was conducted using Metascape.
RESULTS
We identified 488 DEGs between patients with ESCC with distinct prognoses that were mainly enriched in the human immune response, fibrinogen complex, and protein activation cascade pathways. Among patients with ESCC treated with postoperative chemotherapy, those with a high infiltration level of myeloid-derived suppressor cells (MDSCs) had longer overall survival (OS), and OS was positively correlated with the infiltration level of T helper type 2 (Th2) cells among patients treated without chemotherapy after surgery. Additionally, in the case of MDSCs >0.7059 or Th2 cells <0.6290, patients receiving postoperative chemotherapy had a longer OS than those treated without chemotherapy following surgery.
CONCLUSION
The level of MDSCs or Th2 cells can be used as a biomarker for assessing the prognosis of patients with ESCC treated with or without postoperative chemotherapy, respectively.
目的
通过分析患者遗传变异和肿瘤浸润淋巴细胞（TILs）浸润水平，鉴定与食管鳞状细胞癌（ESCC）预后相关的生物标志物。
方法
收集 61 例 ESCC 患者的临床特征。进行 DNA 面板测序以筛选差异表达基因（DEGs）。进行转录组测序以鉴定基因表达谱，并使用 Metascape 对 DEGs 进行后续富集分析。
结果
我们在预后不同的 ESCC 患者之间鉴定了 488 个 DEGs，这些基因主要富集在人类免疫反应、纤维蛋白原复合物和蛋白激活级联途径中。在接受术后化疗的 ESCC 患者中，髓系来源抑制细胞（MDSCs）浸润水平较高的患者总生存期（OS）较长，且未接受术后化疗的患者中 Th2 细胞浸润水平与 OS 呈正相关。此外，当 MDSCs＞0.7059 或 Th2 细胞＜0.6290 时，接受术后化疗的患者 OS 长于未接受术后化疗的患者。
结论
MDSCs 或 Th2 细胞水平可作为评估接受或不接受术后化疗的 ESCC 患者预后的生物标志物。
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