A Novel Infection-Related Inflammatory Response-Related Genes Signature Predicts the Prognosis of Esophageal Squamous Cell Carcinoma.

BACKGROUND

Our previous research showed that () infection can activate the inflammatory signaling pathway and promotes the malignancy development of esophageal squamous cell carcinoma (ESCC). However, the prognostic significance of inflammatory response-related genes (IRRGs) in -infected ESCC requires further elucidation. Hence, our study constructed a prognostic signature based on and IRRGs to forecast the survival of patients with ESCC, which may provide insight into new treatment options for ESCC patients.

METHODS

Differentially expressed genes (DEGs) were identified in -infected and -uninfected ESCC cell by RNA sequencing. A risk model was constructed and validated using the The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database by using univariate Cox regression analysis, LASSO, and the multivariate Cox regression analysis. Kaplan-Meier analysis was carried out to compare the overall survival (OS) between high-risk and low-risk groups. Single-sample gene set enrichment analysis was used to analyze the immune cell infiltration. The Genomics of Drug Sensitivity in Cancer database was used to predict drug sensitivity.

RESULTS

There were 365 DEGs between the -infected and -uninfected groups. Four genes including DKK1, ESRRB, EREG, and RELN were identified to construct the prognostic risk model ( = .012, C-index = 0.73). In both the training and validation sets, patients had a considerably shorter OS in the high-risk group than those in the low-risk group ( < .05). A nomogram was established using the risk score, gender, and N stage which could effectively forecast the prognosis of patients ( = .016, C-index = 0.66). The high-risk group displayed lower immune infiltrating cells, such as activated dendritic cells, type 2 T helper cells, and neutrophils ( < .05). A total of 41 drugs, including dactinomycin, luminespib, and sepantronium bromide, had a significant difference in IC50 between the 2 subgroups.

CONCLUSION

We demonstrated the potential of a novel signature constructed from 4 -related IRRGs for prognostic prediction in ESCC patients.