Nanjing Tongren Hospital, School of Medicine, Southeast University, Nanjing, 211102, China.
Nanjing Kingmed Center for Clinical Laboratory Co., Ltd, Nanjing, 210043, China.
BMC Infect Dis. 2021 May 27;21(1):492. doi: 10.1186/s12879-021-06186-5.
Group B streptococcus (GBS) is the leading cause of early-onset neonatal sepsis. However, GBS was infrequently reported in the developing world in contrast to western countries. This study assessed the prevalence of GBS colonization among pregnant women in Jiangsu, East China, and revealed the difference of GBS infection between culture and PCR.
A total of 16,184 pregnant women at 34 to 37 weeks' gestation aged 16-47 years were recruited from Nanjing Kingmed Center for Clinical Laboratory. Nine thousand twenty-two pregnant women received GBS screening by PCR detection only. Seven thousand one hundred sixty-two pregnant women received GBS screening by bacterial culture and GBS-positive samples were tested for antibiotic resistance.
The overall GBS positive rate was 8.7% by PCR and 3.5% by culture. Colonization rate was highest in the "25-29 years" age group. The 249 GBS-positive samples which detected by culture were all sensitive to penicillin. The prevalence of resistance to erythromycin, clindamycin, and levofloxacin was 77.5, 68.3, and 52.2%, respectively.
This study revealed the data on the prevalence of GBS colonization in pregnant women at 34 to 37 weeks' gestation in Jiangsu, East China. It compared the difference of the sensitivity to detect GBS between PCR and culture. PCR was expected to become a quick method in pregnancy women conventional detection of GBS infection.
B 群链球菌（GBS）是导致早发性新生儿败血症的主要原因。然而，与西方国家相比，发展中国家很少报道 GBS。本研究评估了华东地区江苏省孕妇中 GBS 定植的流行情况，并揭示了培养和 PCR 检测之间 GBS 感染的差异。
共招募了 16184 名年龄在 16-47 岁、妊娠 34-37 周的南京金域临床检验中心孕妇。9022 名孕妇仅接受 PCR 检测的 GBS 筛查。7162 名孕妇接受细菌培养的 GBS 筛查，GBS 阳性样本进行抗生素耐药性检测。
PCR 检测的总 GBS 阳性率为 8.7%，培养法为 3.5%。以“25-29 岁”年龄组的定植率最高。培养法检测的 249 份 GBS 阳性样本均对青霉素敏感。对红霉素、克林霉素和左氧氟沙星的耐药率分别为 77.5%、68.3%和 52.2%。
本研究揭示了华东地区妊娠 34-37 周孕妇 GBS 定植的流行数据。比较了 PCR 和培养法检测 GBS 的敏感性差异。PCR 有望成为孕妇常规检测 GBS 感染的快速方法。
