Synthetic Biology Group, Research and Development Centre, Reliance Industries Limited, India.
Synthetic Biology Group, Research and Development Centre, Reliance Industries Limited, India.
Carbohydr Polym. 2021 Aug 15;266:118176. doi: 10.1016/j.carbpol.2021.118176. Epub 2021 May 7.
Bacterial nanocellulose production is gaining popularity owing to its applications in food, cosmetics and medical industry. Three Acetobacter strains isolated from organic waste and fermented tea were identified using 16S rDNA sequencing and their ability to produce nanocellulose was studied. Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T. This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied. 1% glycerol was found to be optimal concentration, with up to 69% of the utilized carbon converted to nanocellulose. Maximum productivity of 4.5 g/L of bacterial nanocellulose was obtained. Average nitrogen and phosphorus consumption rate was 45 mg/L/day each. Physical properties such as crystallinity, fibril dimensions, and glass transition temperature were studied. Bacterial cellulose was 80% crystalline when glycerol and glucose were used as carbon source and 73% for fructose and sucrose. Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.
由于其在食品、化妆品和医疗行业的应用，细菌纳米纤维素的生产越来越受欢迎。从有机废物和发酵茶中分离出的三株醋杆菌菌株，通过 16S rDNA 测序进行鉴定，并研究了它们生产纳米纤维素的能力。从康普茶中分离出的菌株与 Komagataeibacter rhaeticus DSM 16663T 的同源性为 99%。这是首次报道该菌株在不同碳源（如葡萄糖、甘油、果糖和蔗糖）下的纳米纤维素生产力的研究。发现 1%的甘油是最佳浓度，高达 69%的利用碳转化为纳米纤维素。获得了 4.5g/L 的细菌纳米纤维素的最大生产率。平均氮和磷的消耗速率分别为每天 45mg/L。对结晶度、原纤尺寸和玻璃化转变温度等物理性质进行了研究。当甘油和葡萄糖作为碳源时，细菌纤维素的结晶度为 80%，果糖和蔗糖的结晶度为 73%。具有独特性能的可再生材料，如细菌纤维素，将是未来在化妆品、复合材料和伤口护理领域应用的方向。
