Department of Paper Technology, Indian Institute of Technology, Roorkee, Uttarakhand 247667, India.
Department of Paper Technology, Indian Institute of Technology, Roorkee, Uttarakhand 247667, India.
Carbohydr Polym. 2021 May 15;260:117807. doi: 10.1016/j.carbpol.2021.117807. Epub 2021 Feb 13.
To date, the production of bacterial nanocellulose (BNC) by standard methods has been well known, while the use of low-cost feedstock as an alternative medium still needs to be explored for BNC commercialization. This study explores the prospect for the use of the different aqueous extract of fruit peel wastes (aE-FPW) as a nutrient and carbon source for the production of BNC. Herein, this objective was accomplished by the use of a novel, high- yielding strain, isolated from rotten apple and further identified as Komagataeibacter xylinus IITR DKH20 using 16 s rRNA sequencing analysis. The physicochemical properties of BNC matrix collected from the various aE-FPW mediums were similar or advanced to those collected with the HS medium. Statistical optimization of BNC based on Central Composite Design was performed to study the effect of significant parameters and the results demonstrated that the BNC yield (11.44 g L) was increased by 4.5 fold after optimization.
迄今为止，标准方法生产细菌纳米纤维素（BNC）已广为人知，而利用低成本原料作为替代培养基仍需要探索用于 BNC 的商业化。本研究探讨了不同水果果皮水提物（aE-FPW）作为生产 BNC 的营养和碳源的应用前景。在此，通过使用一种新型、高产的菌株来实现这一目标，该菌株从腐烂的苹果中分离出来，并通过 16s rRNA 测序分析进一步鉴定为 Komagataeibacter xylinus IITR DKH20。从各种 aE-FPW 培养基中收集的 BNC 基质的物理化学性质与 HS 培养基相似或更优。基于中心组合设计对 BNC 进行了统计学优化，以研究显著参数的影响，结果表明，优化后 BNC 的产量（11.44g/L）增加了 4.5 倍。
