Beijing Key Laboratory of Plant Resources Research and Development, College of Chemistry and Meterials Engineering, Beijing Technology and Business University, Beijing, 100048, China.
Sci Rep. 2021 May 27;11(1):11176. doi: 10.1038/s41598-021-90186-7.
We have recently shown that both UVB and BaP can induce the production of ROS, apoptosis and even cancer. However, the differences in the metabolic profiles of skin damaged by UVB, BaP or UVB combined with BaP have not been studied. Therefore, we examined the metabolic changes in the human foreskin fibroblast injured by UVB or BaP or the combination of the two, using ultra performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (qTOF-MS). 24 metabolites were altered in the UVB damage group, 25 in the BaP damage group, and 33 in the UVB combined with BaP group. These alterations indicated that the metabolic mechanisms of HFF-1 cells treated with UVB or BaP are related to multiple main metabolites including glycerophosphocholine (PC), lactosylceramide (LacCer), guanidinosuccinic acid (GSA), glutathione(GSH), and lysophosphatidylcholine (LysoPC) and the main mechanisms involved glycerophospholipid and glutathione metabolism. Thus, our report provided useful insight into the underlying mechanisms of UVB and BaP damage to skin cells.
我们最近发现，UVB 和 BaP 均可诱导活性氧（ROS）产生、细胞凋亡甚至癌症。然而，尚未研究由 UVB、BaP 或 UVB 与 BaP 联合所致皮肤损伤的代谢特征差异。因此，我们采用超高效液相色谱（UPLC）与四极杆飞行时间质谱（qTOF-MS）联用的方法，检测了 UVB 或 BaP 或两者联合所致人包皮成纤维细胞损伤的代谢变化。在 UVB 损伤组中，有 24 种代谢物发生改变，BaP 损伤组中有 25 种，UVB 联合 BaP 组中有 33 种。这些变化表明，UVB 或 BaP 处理的 HFF-1 细胞的代谢机制与包括甘油磷酸胆碱（PC）、乳糖基神经酰胺（LacCer）、胍基琥珀酸（GSA）、谷胱甘肽（GSH）和溶血磷脂酰胆碱（LysoPC）在内的多种主要代谢物有关，主要涉及甘油磷脂和谷胱甘肽代谢。因此，我们的报告为 UVB 和 BaP 损伤皮肤细胞的潜在机制提供了有用的见解。
