Division of Food and Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, Korea.
Food Science and Human Nutrition, Citrus Research and Education Center, University of Florida, Lake Alfred, Florida.
J Food Biochem. 2019 Feb;43(2):e12701. doi: 10.1111/jfbc.12701. Epub 2018 Oct 24.
In this study, the protective effects of caffeic acid (CA) and sinapic acid (SA) on photoaging in human skin fibroblasts induced by UVB irradiation (30 mJ/cm ) were examined. The results revealed that exposure to UVB irradiation reduced cell viability by approximately 33% compared with the non-UVB irradiated control. However, CA and SA blocked the UVB irradiation-induced cytotoxicity greater than the other phenolic acids. CA and SA also significantly inhibited the release of matrix metalloproteinase 1 (MMP-1) and reduced the expression of MMP-1 mRNA in UVB-irradiated Hs68 cells. Furthermore, CA and SA reduced UVB-induced production of reactive oxygen species (ROS) and collagen degradation in Hs68 cells. Finally, CA and SA effectively downregulated activation of the UVB-induced mitogen-activated protein kinases (MAPKs) and nuclear factor κB (NFκB) signaling pathways. These results imply that hydroxylated cinnamate derivatives can be therapeutic agents against UVB-induced skin photoaging. PRACTICAL APPLICATIONS: Hydroxylated cinnamate derivatives such as CA and SA have a high cytoprotective effect against UV irradiation-induced photoaging in human fibroblasts via the inactivation of the MAPKs/NFκB signaling pathway. The present study suggests that CA and SA may be useful in therapeutic and cosmetic applications for the treatment of skin photoaging.
在这项研究中，研究了咖啡酸（CA）和芥子酸（SA）对 UVB 照射（30 mJ/cm ）诱导的人皮肤成纤维细胞光老化的保护作用。结果表明，与未接受 UVB 照射的对照组相比，暴露于 UVB 照射会使细胞活力降低约 33%。然而，CA 和 SA 阻断了 UVB 照射诱导的细胞毒性，其效果优于其他酚酸。CA 和 SA 还显著抑制了 MMP-1 的释放，并降低了 UVB 照射的 Hs68 细胞中 MMP-1 mRNA 的表达。此外，CA 和 SA 减少了 UVB 诱导的 Hs68 细胞中活性氧（ROS）的产生和胶原蛋白的降解。最后，CA 和 SA 有效地下调了 UVB 诱导的丝裂原活化蛋白激酶（MAPKs）和核因子κB（NFκB）信号通路的激活。这些结果表明，羟基肉桂酸衍生物可以作为治疗 UVB 诱导的皮肤光老化的药物。实际应用：羟基肉桂酸衍生物，如 CA 和 SA，通过使 MAPKs/NFκB 信号通路失活，对人成纤维细胞中由 UV 照射引起的光老化具有高细胞保护作用。本研究表明，CA 和 SA 可能在治疗皮肤光老化的治疗和化妆品应用中有用。
