通过流式细胞术检测嗜碱性粒细胞作为狼疮性肾炎的潜在标志物
Basophils as a potential marker of lupus nephritis by flow cytometry.
作者信息
Jiang Yanni, Chen Jin, Zhao Yi, Liu Yi, Xu Hong, Mo Xianming
机构信息
Department of Rheumatology & Immunology, West China Hospital, Sichuan University, Chengdu, China.
Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University & Collaborative Innovation Center for Biotherapy, Chengdu, China.
出版信息
Future Sci OA. 2021 Feb 16;7(5):FSO690. doi: 10.2144/fsoa-2020-0212.
OBJECTIVE
To establish a convenient and simple flow cytometry immunophenotyping panel to explore immune cellular alterations and potential cellular biomarkers in systemic lupus erythematosus.
MATERIALS AND METHODS
This is a cross-sectional, case-control study including 60 patients with systemic lupus erythematosus and 20 sex- and age-matched healthy controls. A 14-color immunophenotyping panel was applied to detect proportions of circulating immune mononuclear cells, and comparisons between patients and healthy controls, and subgroups of patients, were performed. Correlations between cellular proportions and other parameters were investigated.
RESULTS
After multivariate analysis, significantly decreased proportions of CD4CD8 T cells, natural killer cells and innate lymphoid cells were observed in patients compared with healthy controls. The proportions of basophils were decreased significantly in patients with lupus nephritis (LN) compared with those in patients without LN.
CONCLUSION
In the present study, we found that basophil proportions may be a biomarker of LN.
目的
建立一种简便的流式细胞术免疫表型分析方法，以探索系统性红斑狼疮患者免疫细胞的变化及潜在的细胞生物标志物。
材料与方法
这是一项横断面病例对照研究，纳入60例系统性红斑狼疮患者和20例年龄、性别匹配的健康对照。采用14色免疫表型分析方法检测循环免疫单核细胞比例，并对患者与健康对照以及患者亚组进行比较。研究细胞比例与其他参数之间的相关性。
结果
多因素分析显示，与健康对照相比，患者的CD4CD8 T细胞、自然杀伤细胞和固有淋巴细胞比例显著降低。狼疮性肾炎（LN）患者的嗜碱性粒细胞比例较无LN患者显著降低。
结论
在本研究中，我们发现嗜碱性粒细胞比例可能是LN的一个生物标志物。
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