[系统性红斑狼疮外周血中固有淋巴细胞变化与其临床意义的相关性]
[Correlation between the changes of innate lymphoid cells in peripheral blood of systemic lupus erythematosus and its clinical significance].
作者信息
Chu H, Fang X, Tan Z, Zhen X, Wu R L, Li X P, Wang G S, Wang Y P, Li X M
机构信息
Department of Rheumatology and Immunology, Anhui Provincial Hospital affiliated with Anhui Medical University, Hefei 230001, China.
Centre for Transplantation and Renal Research, Westmead Insitute for Medical Research, the University of Sydney, Westmead 2145 NSW, Australia.
出版信息
Zhonghua Yi Xue Za Zhi. 2019 Jan 15;99(3):169-173. doi: 10.3760/cma.j.issn.0376-2491.2019.03.003.
To explore the function and role of innate lymphoid cells in the pathogenesis of systemic lupus erythematosus (SLE) at different disease activity levels. From Nov 2017 to May 2018, 40 patients with SLE and 15 age-matched healthy non-immune-related diseases controls were enrolled from Anhui provincial hospital. According to the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI)-2K, the patients were divided into active group (20) and remission group (20). The frequency of ILCs, B cells, CD4+T and CD8+T cells from peripheral blood mononuclear cells (PBMCs) was detected by flow cytometry. The subsets of ILCs in each group were compared with the subsets of B cells and T cell respectively. The levels of IL-4, IL-33 and IFN-γ in each group were tested by ELISA. Compared with the control group, ILC1 percentage was significantly increased in SLE active group [(22.33%±2.52%) vs (14.56%±1.28%), 0.018 1]; ILC2 percentage was decreased significantly in both remission group [(19.67%±1.83%) vs (42.48%±3.46%), 0.000 1] and active group [(8.67%±0.83%) vs (19.67%±1.83%), 0.000 1]; ILC3 percentage was decreased significantly in active group [(5.72%±1.08%) vs (14.35%±2.40%), 0.001 3]. SLEDAI score was negatively correlated with the percentage of ILC2 (0.023 9) in all patients. The percentage of ILCs in the remission group (0.046 2) and activity group (0.003 7) were both increased significantly. Moreover, the percentage of ILC2 in active group was negatively correlated with CD4+T cells (0.030 8), and the serum IgG was negatively correlated with ILC2% in all patients (0.013 8). Compared with control group or remission group, the levels of IFN-γ (10.91, 0.000 1) and IL-4 (6.046, 0.004 7) in active group were remarkable higher. However, IL-33 was significantly reduced in active group (6.645, 0.002 7). The percentage of ILC2 (0.154 3, 0.028 8) and ILC3 (0.313 6, 0.001 1) in all patients with SLE were positively correlated with the level of IL-4. The percentage of ILCs is related to disease activity, and ILCs play a "double-edged" role in the pathogenesis of SLE. Its function and mechanism are worth further exploration.
探讨不同疾病活动水平下固有淋巴细胞在系统性红斑狼疮（SLE）发病机制中的作用。2017年11月至2018年5月，从安徽省立医院招募了40例SLE患者和15例年龄匹配的健康非免疫相关疾病对照。根据系统性红斑狼疮疾病活动指数（SLEDAI）-2K，将患者分为活动组（20例）和缓解组（20例）。采用流式细胞术检测外周血单个核细胞（PBMC）中固有淋巴细胞（ILC）、B细胞、CD4⁺T和CD8⁺T细胞的频率。将每组ILC的亚群分别与B细胞和T细胞的亚群进行比较。采用酶联免疫吸附测定法检测每组中白细胞介素-4（IL-4）、白细胞介素-33（IL-33）和干扰素-γ（IFN-γ）的水平。与对照组相比，SLE活动组ILC1百分比显著升高[（22.33%±2.52%）对（14.56%±1.28%），P = 0.018 1]；缓解组[（19.67%±1.83%）对（42.48%±3.46%），P = 0.000 1]和活动组[（8.67%±0.83%）对（19.67%±1.83%），P = 0.000 1]的ILC2百分比均显著降低；活动组ILC3百分比显著降低[（5.72%±1.08%）对（14.35%±2.40%），P = 0.001 3]。在所有患者中，SLEDAI评分与ILC2百分比呈负相关（P = 0.023 9）。缓解组（P = 0.046 2）和活动组（P = 0.003 7）的ILC百分比均显著升高。此外，活动组ILC2百分比与CD4⁺T细胞呈负相关（P = 0.030 8），在所有患者中血清免疫球蛋白G（IgG）与ILC2%呈负相关（P = 0.013 8）。与对照组或缓解组相比，活动组IFN-γ水平（P = 10.91，P = 0.000 1）和IL-4水平（P = 6.046，P = 0.004 7）显著更高。然而，活动组IL-33显著降低（P = 6.645，P = 0.002 7）。在所有SLE患者中，ILC2百分比（P = 0.154 3，P = 0.028 8）和ILC3百分比（P = 0.313 6，P = 0.001 1）与IL-4水平呈正相关。ILC百分比与疾病活动相关，ILC在SLE发病机制中起“双刃剑”作用。其功能和机制值得进一步探索。
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