Cytotoxic effects of hydroalcoholic extract of on PC3 and MCF7 cancer cell lines.

OBJECTIVE

Chemoprevention of cancer by application of natural phytochemical compounds has been used to prevent, delay or suppress cancer progression. a traditional Iranian medicinal herb, has biological properties including anticancer, anti-aging, immuno-stimulatory and antioxidant effects. In this study, anti-proliferative effects of hydroalcoholic extract of on prostate (PC3) and breast (MCF7) cancer cell lines were investigated.

MATERIALS AND METHODS

In the current study, we investigated treatment of PC3 cells with different concentrations of (0, 100, 200, 300, 400, and 500 µg/ml) for 24 and 48 hr; also, MCF7 cells were treated with various concentrations (0-600 µg/ml) of for 48 and 72 hr and cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. mRNA expression of BCL2 Associated X (), B-cell lymphoma 2 (), Cysteine-aspartic proteases () and Phosphatase and tensin homolog () were analyzed by quantitative real-time PCR. Annexin V/PI staining and lactate dehydrogenase (LDH) cytotoxicity assay were used to detect apoptosis.

RESULTS

decreased PC3 and MCF7 cells viability in a dose- and time-dependent manner (p<0.01 to p<0.001). The gene expression of ratio, and increased in -treated cells compared to the control group. induced apoptosis (p<0.001) and LDH activity (p<0.01 to p<0.001).

CONCLUSION

Our findings suggest that extract is able to inhibit proliferation and induce apoptosis in PC3 and MCF7 cell lines. Therefore, extract exerts antitumor activity against cancer cells.