Biochemistry Department, School of Life Sciences, Independent University, Dhaka, 1229, Bangladesh.
Department of Pharmacy, University of Dhaka, Dhaka, 1000, Bangladesh.
J Mol Med (Berl). 2021 Sep;99(9):1293-1309. doi: 10.1007/s00109-021-02088-w. Epub 2021 May 28.
Transporter associated with antigen processing 1 (TAP1) is a transporter protein that represent tumor antigen in the MHC I or HLA complex. Any defect in the TAP1 gene resulting in inadequate tumor tracking. TAP1 influences multidrug resistance (MDR) in human cancer cell lines and hinders the treatment during chemotherapeutic. The association of TAP1 in cancer progression remains mostly unknown and further study of the gene in relation with cancer need to conduct. Thus, the study has designed to analyze the association between the TAP1 with cancer by computationally. The expression pattern of the gene has determined by using ONCOMINE, GENT2, and GEPIA2 online platforms. The protein level of TAP1 was examined by the help of Human Protein Atlas. Samples with different clinical outcomes were investigated to evaluate the expression and promoter methylation in cancer vs. normal tissues by using UALCAN server. The copy number alteration, mutation frequency, and expression level of the gene in different cancer were analyzed by using cBioPortal server. The PrognoScan and KM plotter platforms were used to perform the survival analysis and represented graphically. Additionally, pathway and gene ontology (GO) features correlated to the TAP1 gene were analyzed and presented by bar charts. After arranging the data in a single panel like correlating expression to prognosis, mutational and alterations characteristic, and pathways analysis, we observed some interesting insights that emphasized the importance of the gene in cancer progression. The study found the relationship between the TAP1 expression pattern and prognosis in different cancer tissues and shows how TAP1 affects the clinical characteristics. The analytical data presented in the study is vital to learn about the effect of TAP1 in tumor tissue, where previously studies showing contradicting expression of TAP1 in cancer tissue. The analyzed data can also be utilized further to evade the threats against chemotherapy. Overall, the study provided a new aspect to consider the role of TAP1 gene in cancer progression and survival status. KEY MESSAGES: • This study demonstrated, for the first time, a correlation between the TAP1 gene and tumor progression. • An upregulation of TAP1 mRNA was demonstrated in various cancer types. • This study reported a significant negative correlation for TAP1 gene expression and the survival rate in different cancer types.
抗原加工相关转运蛋白 1（TAP1）是一种转运蛋白，它在 MHC I 或 HLA 复合物中代表肿瘤抗原。TAP1 基因的任何缺陷都会导致肿瘤追踪不足。TAP1 影响人癌细胞系中的多药耐药（MDR），并阻碍化疗期间的治疗。TAP1 在癌症进展中的相关性在很大程度上尚不清楚，需要进一步研究该基因与癌症的关系。因此，本研究旨在通过计算方法分析 TAP1 与癌症之间的相关性。使用 ONCOMINE、GENT2 和 GEPIA2 在线平台确定基因的表达模式。通过 Human Protein Atlas 检查 TAP1 的蛋白水平。使用 UALCAN 服务器研究不同临床结局的样本，以评估癌症与正常组织中的表达和启动子甲基化。使用 cBioPortal 服务器分析不同癌症中基因的拷贝数改变、突变频率和表达水平。使用 PrognoScan 和 KM plotter 平台进行生存分析并以图形方式表示。此外，还通过条形图分析与 TAP1 基因相关的途径和基因本体（GO）特征。将数据整理在一个面板中，例如将表达与预后、突变和改变特征以及途径分析相关联，我们观察到一些有趣的见解，强调了该基因在癌症进展中的重要性。本研究发现 TAP1 表达模式与不同癌症组织中的预后之间的关系，并展示了 TAP1 如何影响临床特征。研究中呈现的分析数据对于了解 TAP1 在肿瘤组织中的作用至关重要，因为之前的研究表明 TAP1 在癌症组织中的表达存在矛盾。分析数据还可进一步用于避免化疗的威胁。总的来说，本研究为考虑 TAP1 基因在癌症进展和生存状态中的作用提供了一个新的方面。
• 本研究首次证明了 TAP1 基因与肿瘤进展之间存在相关性。
• 各种癌症类型中 TAP1 mRNA 的上调。
• 本研究报告了不同癌症类型中 TAP1 基因表达与生存率之间存在显著负相关。
