Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 211100, China.
Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, 211100, China.
Mikrochim Acta. 2021 May 28;188(6):207. doi: 10.1007/s00604-021-04868-0.
A nanozyme based on graphene oxide modified with FeO NPs, CuO NPs, and cucurbit[6]uril has been successfully fabricated by a simple sonochemical technique. By employing CB[6] as a specific binding pocket and FeO@CuO-GO as a peroxidase mimic, this novel nanozyme (BN I) is equipped with molecular recognition ability and enhanced peroxidase-like activity. On the basis of the inhibition effect of homocysteine (Hcy) towards the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) catalyzed by BN I, a simple colorimetric method is established for the sensitive and selective determination of Hcy. This proposed method displays a good linear response in the range 5-200 μM with a detection limit of 1.8 μM. In the practical assay of human plasma samples, the relative standard deviations (RSD) are lower than 11% and the recoveries are between 98.0 and 104.9%. In the assay of human urine samples, the RSD are below 9.0% and the recoveries range from 94.0 to 103.5%. The colorimetric method presented offers a convenient and accurate way for the determination of biomarkers in point-of-care testing (POCT).
一种基于氧化石墨烯修饰的 FeO NPs、CuO NPs 和瓜环的纳米酶，通过简单的超声化学技术成功制备。通过将 CB[6] 作为特定的结合口袋和 FeO@CuO-GO 作为过氧化物酶模拟物，这种新型纳米酶（BN I）具有分子识别能力和增强的过氧化物酶样活性。基于同型半胱氨酸（Hcy）对 BN I 催化的 3,3',5,5'-四甲基联苯胺（TMB）氧化的抑制作用，建立了一种用于灵敏和选择性测定 Hcy 的简单比色法。该方法在 5-200 μM 范围内表现出良好的线性响应，检测限为 1.8 μM。在人血浆样品的实际测定中，相对标准偏差（RSD）低于 11%，回收率在 98.0%至 104.9%之间。在人尿样的测定中，RSD 低于 9.0%，回收率范围在 94.0%至 103.5%之间。所提出的比色法为即时检测（POCT）中生物标志物的测定提供了一种方便、准确的方法。
