State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, P. R. China.
Department of Surgery, The Ohio State University, Columbus, OH, USA.
Methods Mol Biol. 2021;2321:161-168. doi: 10.1007/978-1-0716-1488-4_14.
Group 2 innate lymphoid cells (ILC2), despite their scarcity, are the dominant innate lymphoid cell population in the lung, orchestrating innate immunity and adaptive immunity (Germain and Huang. Curr Opin Immunol 56:76-81, 2019; Krabbendam et al. Immunol Rev 286:74-85, 2018; Mindt et al. Front Immunol 9:840, 2018) . Recent studies reveal that ILC2 play critical roles in inflammation-associated lung injury during sepsis (Lai et al. Cell Death Dis 9:369, 2018; Xu et al. Immunol Cell Biol 96:935-947). Therefore, studies aiming to understand the pathobiology of ILC2 may reveal new therapeutic strategies for sepsis. However, the identification of ILC2 requires multiple surface and intracellular markers. This makes the detection of ILC2 in the lung challenging. Here we describe a method to detect ILC2 in the mouse lung using flow cytometry.
2 型固有淋巴细胞（ILC2）尽管数量较少，但在肺部却是占主导地位的固有淋巴细胞群，可协调固有免疫和适应性免疫（Germain 和 Huang. Curr Opin Immunol 56:76-81, 2019; Krabbendam 等人. Immunol Rev 286:74-85, 2018; Mindt 等人. Front Immunol 9:840, 2018）。最近的研究表明，ILC2 在脓毒症相关的肺部炎症损伤中发挥关键作用（Lai 等人. Cell Death Dis 9:369, 2018; Xu 等人. Immunol Cell Biol 96:935-947）。因此，旨在了解 ILC2 病理生物学的研究可能为脓毒症揭示新的治疗策略。然而，ILC2 的鉴定需要多种表面和细胞内标志物。这使得在肺部检测 ILC2 具有挑战性。在这里，我们描述了一种使用流式细胞术检测小鼠肺部 ILC2 的方法。
