Tott Szymon, Grosicki Marek, Glowacz Jacek, Mohaissen Tasnim, Wojnar-Lason Kamila, Chlopicki Stefan, Baranska Malgorzata
Jagiellonian University, Faculty of Chemistry, Gronostajowa 2, 30-387 Kraków, Poland; Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), Bobrzynskiego 14, 30-348 Krakow, Poland.
Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), Bobrzynskiego 14, 30-348 Krakow, Poland.
Biochim Biophys Acta Mol Basis Dis. 2021 Sep 1;1867(9):166180. doi: 10.1016/j.bbadis.2021.166180. Epub 2021 May 25.
Raman spectroscopy is successfully becoming an analytical tool used to characterize alterations in the biochemical composition of cells. In this work, we identify the features of Raman spectra of murine primary endothelial cells (EC) isolated from lungs, heart, liver, brain, kidney and aorta of normal mice, as well as from heart, lung and liver in a murine model of heart failure (HF) in Tgαq44 mice. Primary cells were measured in suspension immediately after their isolation. Raman images showed that isolated primary EC were elliptical or circular, and did not show organ-specific spectral features for any of the studied organ, i.e. lungs, heart, liver, brain, kidney and aorta. Principal Component Analysis pairwise analysis of primary endothelial cells from FVB mice and Tgαq44 mice revealed an increased protein content in EC isolated from the heart and increased lipid content in EC isolated from the lung in Tgαq44 mice. No significant differences were found in the EC isolated from the liver using the same chemometric procedure. To our knowledge, this is the first report in which Raman spectroscopy has been used to characterize the biochemical phenotype of primary murine EC with developing HF. This pilot study shows that Raman-based analysis of freshly isolated primary EC did not revealed organ-specific features, however disease-associated changes were found in the coronary and pulmonary EC in the early stage of heart failure in Tgαq44 mice.
拉曼光谱正成功地成为一种用于表征细胞生化组成变化的分析工具。在这项研究中，我们确定了从正常小鼠的肺、心脏、肝脏、脑、肾和主动脉以及Tgαq44小鼠心力衰竭（HF）模型的心脏、肺和肝脏中分离出的小鼠原代内皮细胞（EC）的拉曼光谱特征。原代细胞在分离后立即以悬浮状态进行测量。拉曼图像显示，分离出的原代EC呈椭圆形或圆形，并且对于任何所研究的器官，即肺、心脏、肝脏、脑、肾和主动脉，均未显示出器官特异性光谱特征。对FVB小鼠和Tgαq44小鼠的原代内皮细胞进行主成分分析成对分析发现，Tgαq44小鼠中从心脏分离出的EC中蛋白质含量增加，从肺分离出的EC中脂质含量增加。使用相同的化学计量程序，在从肝脏分离出的EC中未发现显著差异。据我们所知，这是首次使用拉曼光谱对患有进展性HF的原代小鼠EC的生化表型进行表征的报告。这项初步研究表明，基于拉曼光谱对新鲜分离的原代EC的分析未揭示器官特异性特征，然而在Tgαq44小鼠心力衰竭早期的冠状动脉和肺EC中发现了与疾病相关的变化。
