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多光谱拉曼区分体外恶性皮肤肿瘤：寻找特异性生物标志物和最佳波长。

Multispectral Raman Differentiation of Malignant Skin Neoplasms In Vitro: Search for Specific Biomarkers and Optimal Wavelengths.

机构信息

Lebedev Physical Institute, 119991 Moscow, Russia.

Semashko National Research Institute of Public Health, 105064 Moscow, Russia.

出版信息

Int J Mol Sci. 2023 Sep 29;24(19):14748. doi: 10.3390/ijms241914748.

DOI:10.3390/ijms241914748
PMID:37834196
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10572672/
Abstract

Confocal scanning Raman and photoluminescence (PL) microspectroscopy is a structure-sensitive optical method that allows the non-invasive analysis of biomarkers in the skin tissue. We used it to perform in vitro diagnostics of different malignant skin neoplasms at several excitation wavelengths (532, 785 and 1064 nm). Distinct spectral differences were noticed in the Raman spectra of basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), compared with healthy skin. Our analysis of Raman/PL spectra at the different excitation wavelengths enabled us to propose two novel wavelength-independent spectral criteria (intensity ratios for 1302 cm and 1445 cm bands, 1745 cm and 1445 cm bands), related to the different vibrational "fingerprints" of cell membrane lipids as biomarkers, which was confirmed by the multivariate curve resolution (MCR) technique. These criteria allowed us to differentiate healthy skin from BCC and SCC with sensitivity and specificity higher than 95%, demonstrating high clinical importance in the differential diagnostics of skin tumors.

摘要

共焦扫描拉曼和光致发光（PL）显微光谱学是一种结构敏感的光学方法，可实现皮肤组织中生物标志物的非侵入性分析。我们使用它在几个激发波长（532、785 和 1064nm）下对不同的恶性皮肤肿瘤进行体外诊断。与健康皮肤相比，基底细胞癌（BCC）和鳞状细胞癌（SCC）的拉曼光谱显示出明显的光谱差异。我们对不同激发波长下的拉曼/PL 光谱进行分析，提出了两个新的波长无关的光谱标准（1302cm 和 1445cm 波段、1745cm 和 1445cm 波段的强度比），这些标准与细胞膜脂质的不同振动“指纹”相关，作为生物标志物，通过多元曲线分辨（MCR）技术得到了证实。这些标准能够以高于 95%的灵敏度和特异性将健康皮肤与 BCC 和 SCC 区分开来，在皮肤肿瘤的鉴别诊断中具有重要的临床意义。

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