采用高效液相色谱-串联质谱法测定水产及其制品中 8 种生物胺，无需衍生化。
Determination of 8 biogenic amines in aquatic products and their derived products by high-performance liquid chromatography-tandem mass spectrometry without derivatization.
机构信息
Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China.
NHC Key Laboratory of Food Safety Risk Assessment, Food Safety Research Unit (2019RU014) of Chinese Academy of Medical Science, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
出版信息
Food Chem. 2021 Nov 1;361:130044. doi: 10.1016/j.foodchem.2021.130044. Epub 2021 May 11.
A method for the determination of 8 biogenic amines in aquatic products and their derived products was established by HPLC-MS/MS without derivatization. The samples were extracted by 5% perchloric acid solution. N-hexane was used to clean the extract. The analytes were separated by a column of ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 µm), and gradient eluted with a mixed solution of (0.5% formic acid) and acetonitrile. Good linearity was obtained with correlation coefficients (R) >0.99. This method achieved higher sensitivity (from 0.1 mg/kg for tyramine, 2-phenylethylamine and tryptamine to 1.0 mg/kg for spermidine, spermine, cadaverin, histamine and putrescine). The average recoveries were demonstrated in the range of 70.9%-113.1%, with relative standard deviations (RSDs) from 0.33% to 10.81%. This method was suitable for the detection of BAs in aquatic products and their products.
建立了一种无需衍生化的 HPLC-MS/MS 法测定水产品及其衍生制品中 8 种生物胺的方法。样品用 5%高氯酸溶液提取，正己烷净化。采用 ACQUITY UPLC HSS T3（100mm×2.1mm，1.8μm）柱分离，以（0.5%甲酸）和乙腈的混合溶液进行梯度洗脱。分析物的相关性系数（R）均大于 0.99，呈现良好的线性关系。该方法的灵敏度较高（从 tyramine、2-phenylethylamine 和 tryptamine 的 0.1mg/kg 到 spermidine、spermine、cadaverine、histamine 和 putrescine 的 1.0mg/kg）。平均回收率在 70.9%-113.1%范围内，相对标准偏差（RSD）为 0.33%-10.81%。该方法适用于水产品及其制品中生物胺的检测。
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